Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4941 and PP_4942 overlap by 4 nucleotides PP_4942 and PP_4943 overlap by 1 nucleotides PP_4943 and PP_4944 overlap by 17 nucleotides
PP_4941: PP_4941 - conserved exported protein of unknown function, at 5,622,334 to 5,623,737
_4941
PP_4942: PP_4942 - conserved protein of unknown function, at 5,623,734 to 5,624,630
_4942
PP_4943: PP_4943 - putative Glycosyl transferase, at 5,624,630 to 5,625,760
_4943
PP_4944: PP_4944 - Carbamoyltransferase, at 5,625,744 to 5,627,501
_4944
Position (kb) 5624
5625
5626 Strain fitness (log2 ratio) -1
0
1
2
3 at 5623.745 kb on - strand at 5623.745 kb on - strand at 5623.775 kb on + strand at 5623.775 kb on + strand at 5623.776 kb on - strand at 5623.776 kb on - strand at 5623.777 kb on + strand at 5623.777 kb on + strand at 5623.921 kb on + strand, within PP_4942 at 5624.046 kb on - strand, within PP_4942 at 5624.098 kb on + strand, within PP_4942 at 5624.098 kb on + strand, within PP_4942 at 5624.098 kb on + strand, within PP_4942 at 5624.099 kb on - strand, within PP_4942 at 5624.142 kb on + strand, within PP_4942 at 5624.585 kb on + strand at 5624.618 kb on + strand at 5624.962 kb on - strand, within PP_4943 at 5625.262 kb on - strand, within PP_4943 at 5625.457 kb on - strand, within PP_4943 at 5625.639 kb on - strand, within PP_4943 at 5625.809 kb on - strand at 5625.944 kb on - strand, within PP_4944 at 5625.959 kb on - strand, within PP_4944 at 5626.598 kb on - strand, within PP_4944 at 5626.664 kb on - strand, within PP_4944 at 5626.664 kb on - strand, within PP_4944 at 5626.664 kb on - strand, within PP_4944 at 5626.730 kb on - strand, within PP_4944 at 5626.730 kb on - strand, within PP_4944 at 5626.730 kb on - strand, within PP_4944
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 5,623,745 - +2.4 5,623,745 - +3.4 5,623,775 + -0.4 5,623,775 + +3.4 5,623,776 - +0.5 5,623,776 - -0.1 5,623,777 + -0.4 5,623,777 + -0.7 5,623,921 + PP_4942 0.21 +0.2 5,624,046 - PP_4942 0.35 +2.4 5,624,098 + PP_4942 0.41 -0.1 5,624,098 + PP_4942 0.41 +1.9 5,624,098 + PP_4942 0.41 -0.8 5,624,099 - PP_4942 0.41 +0.5 5,624,142 + PP_4942 0.45 +2.4 5,624,585 + +0.1 5,624,618 + +1.4 5,624,962 - PP_4943 0.29 +0.1 5,625,262 - PP_4943 0.56 +3.0 5,625,457 - PP_4943 0.73 +0.4 5,625,639 - PP_4943 0.89 +2.4 5,625,809 - +0.8 5,625,944 - PP_4944 0.11 +0.4 5,625,959 - PP_4944 0.12 +0.3 5,626,598 - PP_4944 0.49 +1.4 5,626,664 - PP_4944 0.52 +2.4 5,626,664 - PP_4944 0.52 +0.9 5,626,664 - PP_4944 0.52 +1.4 5,626,730 - PP_4944 0.56 +0.9 5,626,730 - PP_4944 0.56 +2.4 5,626,730 - PP_4944 0.56 +2.4
Or see this region's nucleotide sequence