Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4931 and PP_4932 overlap by 4 nucleotides PP_4932 and PP_4933 are separated by 58 nucleotides PP_4933 and PP_4934 are separated by 81 nucleotides
PP_4931: PP_4931 - conserved protein of unknown function, at 5,611,007 to 5,612,182
_4931
PP_4932: PP_4932 - putative D-arabinose 1-dehydrogenase, at 5,612,179 to 5,612,991
_4932
PP_4933: PP_4933 - conserved protein of unknown function, at 5,613,050 to 5,614,018
_4933
PP_4934: PP_4934 - heptose 7-phosphate kinase/heptose 1-phosphate adenylyltransferase, at 5,614,100 to 5,615,521
_4934
Position (kb)
5613
5614
5615 Strain fitness (log2 ratio)
-1
0
1
2 at 5612.995 kb on - strand at 5612.995 kb on - strand at 5613.096 kb on + strand at 5613.097 kb on - strand at 5613.097 kb on - strand at 5613.097 kb on - strand at 5613.097 kb on - strand at 5613.097 kb on - strand at 5613.206 kb on - strand, within PP_4933 at 5613.351 kb on + strand, within PP_4933 at 5613.351 kb on + strand, within PP_4933 at 5613.351 kb on + strand, within PP_4933 at 5613.352 kb on - strand, within PP_4933 at 5613.571 kb on - strand, within PP_4933 at 5613.571 kb on - strand, within PP_4933 at 5613.590 kb on + strand, within PP_4933 at 5613.616 kb on - strand, within PP_4933 at 5613.626 kb on + strand, within PP_4933 at 5613.626 kb on + strand, within PP_4933 at 5613.626 kb on + strand, within PP_4933 at 5613.627 kb on - strand, within PP_4933 at 5613.627 kb on - strand, within PP_4933 at 5613.627 kb on - strand, within PP_4933 at 5613.627 kb on - strand, within PP_4933 at 5613.743 kb on + strand, within PP_4933 at 5613.786 kb on - strand, within PP_4933 at 5613.786 kb on - strand, within PP_4933 at 5613.786 kb on - strand, within PP_4933 at 5613.820 kb on + strand, within PP_4933 at 5613.834 kb on - strand, within PP_4933 at 5613.888 kb on + strand, within PP_4933
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 5,612,995 - -1.1 5,612,995 - -1.7 5,613,096 + -1.3 5,613,097 - -0.6 5,613,097 - +0.2 5,613,097 - +0.1 5,613,097 - -0.2 5,613,097 - +0.9 5,613,206 - PP_4933 0.16 -1.6 5,613,351 + PP_4933 0.31 -0.1 5,613,351 + PP_4933 0.31 +0.0 5,613,351 + PP_4933 0.31 -0.4 5,613,352 - PP_4933 0.31 -0.3 5,613,571 - PP_4933 0.54 +0.9 5,613,571 - PP_4933 0.54 +1.9 5,613,590 + PP_4933 0.56 -0.7 5,613,616 - PP_4933 0.58 -0.6 5,613,626 + PP_4933 0.59 +0.6 5,613,626 + PP_4933 0.59 +0.6 5,613,626 + PP_4933 0.59 +1.3 5,613,627 - PP_4933 0.60 -0.5 5,613,627 - PP_4933 0.60 -0.6 5,613,627 - PP_4933 0.60 +0.0 5,613,627 - PP_4933 0.60 +2.0 5,613,743 + PP_4933 0.72 +1.3 5,613,786 - PP_4933 0.76 +1.3 5,613,786 - PP_4933 0.76 +2.4 5,613,786 - PP_4933 0.76 +0.5 5,613,820 + PP_4933 0.79 -0.4 5,613,834 - PP_4933 0.81 +0.6 5,613,888 + PP_4933 0.86 +2.4
Or see this region's nucleotide sequence