Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4896 and PP_4897 overlap by 1 nucleotides PP_4897 and PP_4898 are separated by 11 nucleotides PP_4898 and PP_4899 overlap by 13 nucleotides
PP_4896: PP_4896 - DNA mismatch repair protein MutL, at 5,565,587 to 5,567,485
_4896
PP_4897: PP_4897 - N-acetylmuramoyl-L-alanine amidase, at 5,567,485 to 5,568,915
_4897
PP_4898: PP_4898 - ATPase, at 5,568,927 to 5,569,400
_4898
PP_4899: PP_4899 - ADP-dependent (S)-NAD(P)H-hydrate dehydratase, at 5,569,388 to 5,570,248
_4899
Position (kb) 5567
5568
5569 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5566.547 kb on + strand, within PP_4896 at 5566.548 kb on - strand, within PP_4896 at 5566.548 kb on - strand, within PP_4896 at 5566.713 kb on + strand, within PP_4896 at 5566.713 kb on + strand, within PP_4896 at 5566.713 kb on + strand, within PP_4896 at 5566.713 kb on + strand, within PP_4896 at 5566.714 kb on - strand, within PP_4896 at 5567.208 kb on - strand, within PP_4896 at 5567.216 kb on + strand, within PP_4896 at 5567.217 kb on - strand, within PP_4896 at 5567.217 kb on - strand, within PP_4896 at 5567.512 kb on + strand at 5567.512 kb on + strand at 5567.630 kb on - strand, within PP_4897 at 5567.630 kb on - strand, within PP_4897 at 5567.630 kb on - strand, within PP_4897 at 5567.687 kb on + strand, within PP_4897 at 5567.687 kb on + strand, within PP_4897 at 5567.687 kb on + strand, within PP_4897 at 5567.688 kb on - strand, within PP_4897 at 5567.688 kb on - strand, within PP_4897 at 5567.688 kb on - strand, within PP_4897 at 5568.916 kb on - strand at 5568.916 kb on - strand at 5568.929 kb on - strand at 5568.929 kb on - strand at 5568.936 kb on - strand at 5568.936 kb on - strand at 5568.936 kb on - strand at 5568.936 kb on - strand at 5568.936 kb on - strand at 5568.936 kb on - strand at 5568.938 kb on - strand at 5569.390 kb on - strand at 5569.463 kb on - strand at 5569.687 kb on + strand, within PP_4899 at 5569.688 kb on - strand, within PP_4899 at 5569.688 kb on - strand, within PP_4899 at 5569.690 kb on - strand, within PP_4899 at 5569.734 kb on - strand, within PP_4899 at 5569.734 kb on - strand, within PP_4899 at 5569.786 kb on + strand, within PP_4899 at 5569.787 kb on - strand, within PP_4899 at 5569.787 kb on - strand, within PP_4899 at 5569.894 kb on + strand, within PP_4899 at 5569.895 kb on - strand, within PP_4899 at 5569.895 kb on - strand, within PP_4899 at 5569.895 kb on - strand at 5569.895 kb on - strand, within PP_4899
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 5,566,547 + PP_4896 0.51 -2.8 5,566,548 - PP_4896 0.51 -0.1 5,566,548 - PP_4896 0.51 +0.9 5,566,713 + PP_4896 0.59 +0.3 5,566,713 + PP_4896 0.59 +0.5 5,566,713 + PP_4896 0.59 +0.4 5,566,713 + PP_4896 0.59 +0.4 5,566,714 - PP_4896 0.59 -0.1 5,567,208 - PP_4896 0.85 +0.0 5,567,216 + PP_4896 0.86 -0.4 5,567,217 - PP_4896 0.86 +0.1 5,567,217 - PP_4896 0.86 -0.8 5,567,512 + -0.7 5,567,512 + -0.2 5,567,630 - PP_4897 0.10 +0.4 5,567,630 - PP_4897 0.10 -0.6 5,567,630 - PP_4897 0.10 -0.1 5,567,687 + PP_4897 0.14 -1.6 5,567,687 + PP_4897 0.14 -2.1 5,567,687 + PP_4897 0.14 +0.4 5,567,688 - PP_4897 0.14 +0.4 5,567,688 - PP_4897 0.14 -0.7 5,567,688 - PP_4897 0.14 -2.2 5,568,916 - +0.9 5,568,916 - +2.4 5,568,929 - -2.1 5,568,929 - -0.3 5,568,936 - -1.6 5,568,936 - +0.4 5,568,936 - -0.8 5,568,936 - +0.0 5,568,936 - -1.3 5,568,936 - +1.0 5,568,938 - +0.4 5,569,390 - +0.1 5,569,463 - -0.5 5,569,687 + PP_4899 0.35 -1.8 5,569,688 - PP_4899 0.35 +0.2 5,569,688 - PP_4899 0.35 -0.7 5,569,690 - PP_4899 0.35 +0.1 5,569,734 - PP_4899 0.40 +0.1 5,569,734 - PP_4899 0.40 +0.1 5,569,786 + PP_4899 0.46 +1.0 5,569,787 - PP_4899 0.46 -0.8 5,569,787 - PP_4899 0.46 +0.3 5,569,894 + PP_4899 0.59 +1.1 5,569,895 - PP_4899 0.59 +1.2 5,569,895 - PP_4899 0.59 +1.4 5,569,895 - +2.4 5,569,895 - PP_4899 0.59 +0.4
Or see this region's nucleotide sequence