Strain Fitness in Pseudomonas putida KT2440 around PP_4890

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,559,674	-		PP_4890	0.31	-4.4
5,559,674	-		PP_4890	0.31	-2.6
5,559,992	-		PP_4890	0.57	-1.4
5,560,103	-		PP_4890	0.67	-3.7
5,560,103	-		PP_4890	0.67	-2.1
5,560,103	-		PP_4890	0.67	-3.5
5,560,291	-		PP_4890	0.83	-2.0
5,560,349	-		PP_4890	0.88	-2.8
5,560,349	-		PP_4890	0.88	-3.5
5,560,450	-				-3.3
5,560,486	-				-2.1
5,560,498	-				-0.1
5,560,713	-				-2.0
5,560,713	-				-1.2
5,560,713	-				-1.5
5,560,713	-				-1.5
5,560,713	-				-0.3
5,560,752	+				+0.7
5,560,752	+				+1.4
5,560,886	+		PP_4891	0.10	+2.4
5,560,935	-		PP_4891	0.16	+2.4
5,560,935	-		PP_4891	0.16	+1.2
5,560,946	+		PP_4891	0.17	+2.4
5,560,946	+		PP_4891	0.17	+0.9
5,560,946	+		PP_4891	0.17	+1.4
5,560,946	+		PP_4891	0.17	+2.4
5,560,946	+		PP_4891	0.17	+0.9
5,560,947	-		PP_4891	0.17	-0.1
5,560,947	-		PP_4891	0.17	+1.9
5,560,947	-		PP_4891	0.17	+1.4
5,560,947	-		PP_4891	0.17	+2.4
5,560,950	+		PP_4891	0.17	+1.6
5,560,951	-		PP_4891	0.18	+0.7
5,560,951	-		PP_4891	0.18	-1.5
5,560,951	-		PP_4891	0.18	+0.9
5,561,000	+		PP_4891	0.23	+1.4
5,561,001	-		PP_4891	0.23	+0.7
5,561,278	-		PP_4891	0.55	-0.6
5,561,278	-		PP_4891	0.55	-1.4
5,561,366	+		PP_4891	0.65	+0.9
5,561,367	-		PP_4891	0.65	+2.4
5,561,367	-		PP_4891	0.65	+0.1