Strain Fitness in Pseudomonas putida KT2440 around PP_4739

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,389,842	+		PP_4738	0.76	-0.1
5,389,842	+		PP_4738	0.76	-0.6
5,389,843	-		PP_4738	0.76	-0.4
5,389,843	-		PP_4738	0.76	-0.2
5,389,843	-		PP_4738	0.76	-1.0
5,389,963	+				+2.4
5,389,965	+				+2.4
5,389,984	+				+2.4
5,390,501	+				+0.8
5,390,519	+				-0.3
5,390,519	+				-0.8
5,390,520	-				-0.3
5,390,520	-				-0.3
5,390,520	-				+0.2
5,390,520	-				-0.5
5,390,520	-				+2.0
5,390,565	-				-0.5
5,390,565	-				-0.5
5,390,617	+				+0.7
5,390,617	+				-0.9
5,390,618	-				+0.5
5,390,618	-				+0.2
5,390,631	-				+0.1
5,390,631	-				+0.0
5,390,765	+				+0.5
5,390,765	+				-0.1
5,390,773	+				-0.3
5,390,773	+				-1.7
5,390,878	+		PP_4739	0.45	-1.2
5,390,878	+		PP_4739	0.45	-1.0
5,390,977	+		PP_4739	0.86	+0.3
5,390,977	+		PP_4739	0.86	-0.9
5,391,222	+		PP_5707	0.52	+0.1
5,391,288	+		PP_5707	0.77	-0.0
5,391,288	+		PP_5707	0.77	-1.1
5,391,289	-		PP_5707	0.78	-1.5
5,391,289	-		PP_5707	0.78	-1.0
5,391,289	-		PP_5707	0.78	+1.6
5,391,289	-		PP_5707	0.78	-2.1
5,391,366	+				-0.1
5,391,366	+				-0.9
5,391,366	+				-1.0
5,391,366	+				+0.3
5,391,367	-				+0.3
5,391,453	+		PP_5708	0.21	+2.4
5,391,453	+		PP_5708	0.21	-0.1
5,391,453	+		PP_5708	0.21	-0.2
5,391,454	-		PP_5708	0.22	-1.0
5,391,506	+		PP_5708	0.48	+0.1
5,391,506	+		PP_5708	0.48	-1.5
5,391,506	+		PP_5708	0.48	+0.5
5,391,507	-		PP_5708	0.48	-0.4
5,391,507	-		PP_5708	0.48	-0.3
5,391,507	-		PP_5708	0.48	-0.1
5,391,550	+		PP_5708	0.70	+0.1
5,391,550	+		PP_5708	0.70	+0.4
5,391,566	+		PP_5708	0.78	+0.2
5,391,567	-				+0.1
5,391,595	+				-0.9
5,391,595	+				+1.4
5,391,595	+				-0.3
5,391,596	-				-1.6
5,391,596	-				+0.6
5,391,596	-				+0.1
5,391,598	+				+0.7
5,391,599	-				+0.5
5,391,599	-				-0.2
5,391,672	+		PP_5709	0.17	-0.2
5,391,673	-		PP_5709	0.17	-0.1
5,391,673	-		PP_5709	0.17	-1.5
5,391,723	+		PP_5709	0.36	-0.4
5,391,724	-		PP_5709	0.36	-0.9
5,391,724	-		PP_5709	0.36	+0.5
5,391,724	-		PP_5709	0.36	-0.4
5,391,724	-		PP_5709	0.36	-0.2
5,391,728	+		PP_5709	0.38	+0.4
5,391,729	-		PP_5709	0.38	-0.0
5,391,729	-		PP_5709	0.38	-0.6
5,391,729	-		PP_5709	0.38	-1.1
5,391,751	+		PP_5709	0.46	-0.0
5,391,752	-		PP_5709	0.47	-0.9
5,391,796	-		PP_5709	0.63	-1.6
5,391,796	-		PP_5709	0.63	-0.0
5,391,895	+				+0.7
5,391,916	+				-0.0
5,391,963	+				+1.3