Strain Fitness in Pseudomonas putida KT2440 around PP_4684

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,322,459	-		PP_4683	0.66	-0.7
5,322,486	-		PP_4683	0.67	-0.1
5,322,557	+		PP_4683	0.70	-0.3
5,322,558	-		PP_4683	0.70	-0.5
5,322,558	-		PP_4683	0.70	+0.6
5,322,675	-		PP_4683	0.75	+0.2
5,322,675	-		PP_4683	0.75	+0.3
5,322,675	-		PP_4683	0.75	+0.4
5,322,675	-		PP_4683	0.75	+0.1
5,322,716	+		PP_4683	0.77	+0.0
5,322,716	+		PP_4683	0.77	+0.5
5,322,716	+		PP_4683	0.77	-0.7
5,322,716	+		PP_4683	0.77	-1.1
5,322,717	-		PP_4683	0.77	+0.1
5,322,724	+		PP_4683	0.77	-1.0
5,322,770	+		PP_4683	0.79	-0.2
5,322,900	-		PP_4683	0.85	+1.4
5,322,900	-		PP_4683	0.85	+0.8
5,322,900	-		PP_4683	0.85	+1.1
5,322,934	-		PP_4683	0.86	+2.4
5,322,934	-		PP_4683	0.86	-1.4
5,322,934	-		PP_4683	0.86	-0.8
5,323,064	+				-0.4
5,323,064	+				+1.2
5,323,064	+				+0.4
5,323,065	-				+0.8
5,323,065	-				-1.0
5,323,127	+				+0.3
5,323,127	+				-0.4
5,323,128	-				+0.2
5,323,265	-				-0.9
5,323,265	-				+0.4
5,323,265	-				-0.3
5,323,265	-				-0.3
5,323,273	+				+0.8
5,323,273	+				-0.8
5,323,273	+				-0.7
5,323,273	+				-2.3
5,323,274	-				-0.2
5,323,274	-				+3.0
5,323,276	-				+0.5
5,323,276	-				+0.2
5,323,276	-				-1.4
5,323,326	+				+1.0
5,323,326	+				+2.4
5,323,381	+				-1.2
5,323,381	+				+0.4
5,323,381	+				-0.5
5,323,382	-				+0.4
5,323,382	-				-0.2
5,323,382	-				-0.3
5,323,384	+				+0.8
5,323,409	+				-1.6
5,323,410	-				+2.4
5,323,410	-				-0.5
5,323,450	+				-0.9
5,323,450	+				-1.0
5,323,451	-				+0.6
5,323,451	-				-1.2
5,323,451	-				-1.4
5,323,478	+				-0.7
5,323,484	+				+1.7
5,323,484	+				+0.8
5,323,485	-				-1.7
5,323,485	-				-0.2
5,323,598	+		PP_4684	0.15	+0.8
5,323,670	+		PP_4684	0.19	-0.8
5,323,670	+		PP_4684	0.19	+1.4
5,323,671	-		PP_4684	0.19	-0.5
5,323,671	-		PP_4684	0.19	-2.1
5,324,180	+		PP_4684	0.52	-1.0
5,324,202	-		PP_4684	0.53	-1.3
5,324,228	+		PP_4684	0.55	-1.2
5,324,334	-		PP_4684	0.62	-0.9
5,324,334	-		PP_4684	0.62	-1.2
5,324,354	+		PP_4684	0.63	-0.5
5,324,473	-		PP_4684	0.71	-0.6
5,324,479	+		PP_4684	0.71	+1.4
5,324,535	+		PP_4684	0.75	-1.0
5,324,535	+		PP_4684	0.75	-0.4
5,324,535	+		PP_4684	0.75	+2.4
5,324,535	+		PP_4684	0.75	-2.0
5,324,535	+		PP_4684	0.75	-0.9
5,324,536	-		PP_4684	0.75	-1.2
5,324,537	+		PP_4684	0.75	-0.9
5,324,537	+		PP_4684	0.75	-0.4
5,324,537	+		PP_4684	0.75	+0.1
5,324,537	+		PP_4684	0.75	-1.4
5,324,537	+		PP_4684	0.75	-0.3
5,324,537	+		PP_4684	0.75	-0.2
5,324,537	+		PP_4684	0.75	-0.7
5,324,538	-		PP_4684	0.75	+0.6
5,324,538	-		PP_4684	0.75	-1.3
5,324,538	-		PP_4684	0.75	-1.4
5,324,564	+		PP_4684	0.77	-1.4
5,324,564	+		PP_4684	0.77	+0.4
5,324,565	-		PP_4684	0.77	+0.7
5,324,633	+		PP_4684	0.81	-1.4
5,324,634	-		PP_4684	0.81	-0.6
5,324,927	+				-1.4
5,324,927	+				-1.8
5,324,928	-				+0.4
5,324,928	-				-0.7
5,324,928	-				-0.6
5,324,928	-				-0.8
5,325,049	+				+1.7
5,325,202	+		PP_4685	0.41	-0.3
5,325,203	-		PP_4685	0.41	-0.6
5,325,250	+		PP_4685	0.58	+0.2
5,325,250	+		PP_4685	0.58	-0.2
5,325,250	+		PP_4685	0.58	+0.7
5,325,250	+		PP_4685	0.58	+1.5
5,325,250	+		PP_4685	0.58	-1.5
5,325,251	-		PP_4685	0.58	-0.4
5,325,251	-		PP_4685	0.58	+0.6
5,325,251	-		PP_4685	0.58	-1.2
5,325,251	-		PP_4685	0.58	+0.2
5,325,251	-		PP_4685	0.58	-0.3
5,325,251	-		PP_4685	0.58	-0.4
5,325,294	-		PP_4685	0.74	-1.0
5,325,364	+				+0.3
5,325,443	+				-0.6
5,325,443	+				+0.8
5,325,443	+				-0.2
5,325,443	+				-1.4
5,325,443	+				-1.6
5,325,443	+				-1.6
5,325,444	-				-1.5
5,325,444	-				-0.1
5,325,444	-				+0.1
5,325,444	-				-1.3
5,325,471	+				-1.3
5,325,471	+				-0.6
5,325,471	+				-0.8
5,325,472	-				+1.1
5,325,488	-				-2.0
5,325,488	-				-1.7
5,325,569	+				-0.7
5,325,570	-				+1.4
5,325,570	-				+0.4
5,325,570	-				-0.7
5,325,605	+				+0.3
5,325,605	+				-1.2
5,325,605	+				-0.7
5,325,606	-				-0.2
5,325,606	-				-1.3
5,325,606	-				-0.3
5,325,606	-				+0.4
5,325,606	-				-0.7
5,325,606	-				-1.0
5,325,656	-		PP_4686	0.12	-0.2
5,325,656	-		PP_4686	0.12	-0.3
5,325,700	+		PP_4686	0.17	-1.1
5,325,700	+		PP_4686	0.17	+0.8
5,325,701	-		PP_4686	0.17	-0.2
5,325,725	+		PP_4686	0.20	-0.4
5,325,725	+		PP_4686	0.20	-1.2
5,325,725	+		PP_4686	0.20	-2.0
5,325,725	+		PP_4686	0.20	-0.9
5,325,725	+		PP_4686	0.20	-1.0
5,325,725	+		PP_4686	0.20	-0.9
5,325,726	-		PP_4686	0.20	+0.2
5,325,726	-		PP_4686	0.20	+0.8
5,325,726	-		PP_4686	0.20	+0.4