Strain Fitness in Pseudomonas putida KT2440 around PP_4681

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4680 and PP_4681 are separated by 408 nucleotidesPP_4681 and PP_4682 overlap by 4 nucleotidesPP_4682 and PP_4683 are separated by 15 nucleotides PP_4680: PP_4680 - acetohydroxybutanoate synthase / acetolactate synthase, at 5,317,649 to 5,319,373 _4680 PP_4681: PP_4681 - conserved protein of unknown function, at 5,319,782 to 5,320,117 _4681 PP_4682: PP_4682 - conserved protein of unknown function containing tetratricopeptide repeat domain, at 5,320,114 to 5,320,914 _4682 PP_4683: PP_4683 - transglycosylase/transpeptidase, at 5,320,930 to 5,323,251 _4683 Position (kb) 5319 5320 5321Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 5318.820 kb on + strand, within PP_4680at 5318.821 kb on - strand, within PP_4680at 5318.853 kb on + strand, within PP_4680at 5318.854 kb on - strand, within PP_4680at 5318.881 kb on + strand, within PP_4680at 5318.882 kb on - strand, within PP_4680at 5318.882 kb on - strand, within PP_4680at 5319.013 kb on + strand, within PP_4680at 5319.014 kb on - strand, within PP_4680at 5319.014 kb on - strand, within PP_4680at 5319.061 kb on + strand, within PP_4680at 5319.061 kb on + strand, within PP_4680at 5319.061 kb on + strand, within PP_4680at 5319.061 kb on + strand, within PP_4680at 5319.062 kb on - strand, within PP_4680at 5319.062 kb on - strand, within PP_4680at 5319.062 kb on - strand, within PP_4680at 5319.062 kb on - strand, within PP_4680at 5319.062 kb on - strand, within PP_4680at 5319.105 kb on + strand, within PP_4680at 5319.105 kb on + strand, within PP_4680at 5319.105 kb on + strand, within PP_4680at 5319.106 kb on - strand, within PP_4680at 5319.106 kb on - strand, within PP_4680at 5319.206 kb on - strandat 5319.274 kb on - strandat 5319.306 kb on + strandat 5319.306 kb on + strandat 5319.306 kb on + strandat 5319.307 kb on - strandat 5319.307 kb on - strandat 5319.307 kb on - strandat 5319.307 kb on - strandat 5319.362 kb on + strandat 5319.363 kb on - strandat 5319.386 kb on + strandat 5319.399 kb on + strandat 5319.422 kb on + strandat 5319.422 kb on + strandat 5319.425 kb on + strandat 5319.425 kb on + strandat 5319.426 kb on - strandat 5319.431 kb on + strandat 5319.432 kb on - strandat 5319.432 kb on - strandat 5319.477 kb on + strandat 5319.478 kb on - strandat 5319.543 kb on + strandat 5319.544 kb on - strandat 5319.607 kb on + strandat 5319.830 kb on + strand, within PP_4681at 5319.979 kb on + strand, within PP_4681at 5320.000 kb on + strand, within PP_4681at 5320.001 kb on - strand, within PP_4681at 5320.091 kb on - strandat 5320.236 kb on - strand, within PP_4682at 5320.310 kb on + strand, within PP_4682at 5320.310 kb on + strand, within PP_4682at 5320.482 kb on + strand, within PP_4682at 5320.626 kb on + strand, within PP_4682at 5320.752 kb on + strandat 5320.752 kb on + strand, within PP_4682at 5320.754 kb on + strand, within PP_4682at 5320.755 kb on - strand, within PP_4682at 5320.755 kb on - strand, within PP_4682at 5320.755 kb on - strand, within PP_4682at 5320.932 kb on - strandat 5320.969 kb on + strandat 5320.970 kb on - strandat 5321.027 kb on + strandat 5321.028 kb on - strandat 5321.028 kb on - strandat 5321.028 kb on - strandat 5321.078 kb on + strandat 5321.078 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
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5,318,820 + PP_4680 0.68 +2.4
5,318,821 - PP_4680 0.68 +2.4
5,318,853 + PP_4680 0.70 -2.7
5,318,854 - PP_4680 0.70 -2.2
5,318,881 + PP_4680 0.71 +2.4
5,318,882 - PP_4680 0.71 -2.3
5,318,882 - PP_4680 0.71 -3.3
5,319,013 + PP_4680 0.79 -3.6
5,319,014 - PP_4680 0.79 -3.0
5,319,014 - PP_4680 0.79 -3.7
5,319,061 + PP_4680 0.82 -0.6
5,319,061 + PP_4680 0.82 -2.7
5,319,061 + PP_4680 0.82 -3.6
5,319,061 + PP_4680 0.82 -1.2
5,319,062 - PP_4680 0.82 -3.7
5,319,062 - PP_4680 0.82 -0.7
5,319,062 - PP_4680 0.82 -3.0
5,319,062 - PP_4680 0.82 -0.9
5,319,062 - PP_4680 0.82 +2.4
5,319,105 + PP_4680 0.84 -1.7
5,319,105 + PP_4680 0.84 -3.8
5,319,105 + PP_4680 0.84 -0.7
5,319,106 - PP_4680 0.84 -2.1
5,319,106 - PP_4680 0.84 -0.4
5,319,206 - -2.7
5,319,274 - -4.3
5,319,306 + -3.8
5,319,306 + -2.4
5,319,306 + -3.5
5,319,307 - -1.3
5,319,307 - -2.2
5,319,307 - +1.4
5,319,307 - -1.6
5,319,362 + -2.2
5,319,363 - -2.2
5,319,386 + -2.0
5,319,399 + -3.6
5,319,422 + -3.6
5,319,422 + -3.4
5,319,425 + -0.6
5,319,425 + -3.3
5,319,426 - +0.2
5,319,431 + -4.0
5,319,432 - -0.0
5,319,432 - +0.4
5,319,477 + -2.7
5,319,478 - -1.5
5,319,543 + -3.1
5,319,544 - -1.5
5,319,607 + +1.4
5,319,830 + PP_4681 0.14 -2.7
5,319,979 + PP_4681 0.59 +0.1
5,320,000 + PP_4681 0.65 -0.7
5,320,001 - PP_4681 0.65 -1.4
5,320,091 - +0.1
5,320,236 - PP_4682 0.15 +1.5
5,320,310 + PP_4682 0.24 -1.4
5,320,310 + PP_4682 0.24 -0.8
5,320,482 + PP_4682 0.46 -0.3
5,320,626 + PP_4682 0.64 +0.1
5,320,752 + +2.4
5,320,752 + PP_4682 0.80 +0.2
5,320,754 + PP_4682 0.80 +0.6
5,320,755 - PP_4682 0.80 -0.1
5,320,755 - PP_4682 0.80 -0.3
5,320,755 - PP_4682 0.80 +0.2
5,320,932 - -0.5
5,320,969 + -1.5
5,320,970 - -0.1
5,321,027 + -0.7
5,321,028 - -1.2
5,321,028 - +0.3
5,321,028 - +0.4
5,321,078 + -0.2
5,321,078 + +0.5

Or see this region's nucleotide sequence