Strain Fitness in Pseudomonas putida KT2440 around PP_4678

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,316,015	-				+0.5
5,316,015	-				+1.0
5,316,015	-				+0.7
5,316,015	-				-1.5
5,316,017	-				+0.6
5,316,084	+				+0.7
5,316,084	+				+0.4
5,316,085	-				-0.7
5,316,085	-				+0.3
5,316,085	-				-0.8
5,316,085	-				-1.6
5,316,208	-		PP_4678	0.12	-4.3
5,316,214	+		PP_4678	0.13	-0.2
5,316,215	-		PP_4678	0.13	-2.5
5,316,215	-		PP_4678	0.13	-1.3
5,316,215	-		PP_4678	0.13	-2.1
5,316,215	-		PP_4678	0.13	-2.2
5,316,324	+		PP_4678	0.24	-2.2
5,316,324	+		PP_4678	0.24	-1.2
5,316,324	+		PP_4678	0.24	-3.2
5,316,334	-		PP_4678	0.25	-1.0
5,316,357	+		PP_4678	0.27	-0.9
5,316,357	+		PP_4678	0.27	-1.6
5,316,384	+		PP_4678	0.30	-4.1
5,316,384	+		PP_4678	0.30	-3.5
5,316,384	+		PP_4678	0.30	-3.0
5,316,384	+		PP_4678	0.30	-3.3
5,316,385	-		PP_4678	0.30	-2.8
5,316,385	-		PP_4678	0.30	-2.9
5,316,385	-		PP_4678	0.30	-2.4
5,316,385	-		PP_4678	0.30	-3.0
5,316,395	+		PP_4678	0.31	-4.3
5,316,395	+		PP_4678	0.31	-2.0
5,316,395	+		PP_4678	0.31	-2.5
5,316,396	-		PP_4678	0.31	-2.5
5,316,396	-		PP_4678	0.31	-3.1
5,316,396	-		PP_4678	0.31	-2.8
5,316,429	+		PP_4678	0.34	-1.4
5,316,568	+		PP_4678	0.48	-1.8
5,316,587	+		PP_4678	0.50	-2.2
5,316,587	+		PP_4678	0.50	-3.2
5,316,588	-		PP_4678	0.50	-2.5
5,316,588	-		PP_4678	0.50	+2.4
5,316,600	+		PP_4678	0.51	-0.4
5,316,600	+		PP_4678	0.51	-4.0
5,316,601	-		PP_4678	0.51	-4.2
5,316,642	+		PP_4678	0.55	-3.2
5,316,642	+		PP_4678	0.55	-0.8
5,316,643	-		PP_4678	0.55	-0.2
5,316,643	-		PP_4678	0.55	-0.4
5,316,643	-		PP_4678	0.55	-2.2
5,316,643	-		PP_4678	0.55	-2.7
5,316,646	+		PP_4678	0.55	-1.8
5,316,646	+		PP_4678	0.55	-3.8
5,316,646	+		PP_4678	0.55	-2.7
5,316,762	+		PP_4678	0.67	-1.3
5,316,762	+		PP_4678	0.67	-2.8
5,316,762	+		PP_4678	0.67	-2.9
5,316,834	+		PP_4678	0.74	-2.1
5,316,834	+		PP_4678	0.74	-3.1
5,316,834	+		PP_4678	0.74	-2.9
5,316,835	-		PP_4678	0.74	+0.4
5,316,835	-		PP_4678	0.74	-3.4
5,316,899	-		PP_4678	0.80	-2.5
5,316,958	+		PP_4678	0.86	-3.3
5,316,959	-		PP_4678	0.86	-2.9
5,316,959	-		PP_4678	0.86	-3.9
5,316,959	-		PP_4678	0.86	-2.0
5,316,980	+		PP_4678	0.88	-2.4
5,316,980	+		PP_4678	0.88	-1.6
5,317,051	+				-2.4
5,317,052	-				-2.9
5,317,082	-				-2.0
5,317,115	+				-2.8
5,317,116	-				+2.4
5,317,117	+				-3.5
5,317,117	+				-3.5
5,317,117	+				+0.4
5,317,289	+		PP_4679	0.27	-0.8
5,317,289	+		PP_4679	0.27	-0.8
5,317,290	-		PP_4679	0.27	-1.8
5,317,290	-		PP_4679	0.27	-1.7
5,317,329	+		PP_4679	0.35	-3.2
5,317,330	-		PP_4679	0.35	-2.3
5,317,330	-		PP_4679	0.35	+1.4
5,317,544	-		PP_4679	0.79	-1.8
5,317,565	+		PP_4679	0.83	-1.8
5,317,565	+		PP_4679	0.83	-2.1
5,317,565	+		PP_4679	0.83	+0.1
5,317,566	-		PP_4679	0.83	-0.9
5,317,566	-		PP_4679	0.83	-2.3
5,317,566	-		PP_4679	0.83	-1.8
5,317,566	-		PP_4679	0.83	-0.8
5,317,566	-		PP_4679	0.83	+1.4
5,317,654	+				-2.8
5,317,654	+				-3.6
5,317,654	+				-2.7
5,317,654	+				-3.6
5,317,654	+				-2.3
5,317,713	+				-2.9
5,317,836	+		PP_4680	0.11	-2.5
5,317,836	+		PP_4680	0.11	-2.7
5,317,836	+		PP_4680	0.11	-3.2
5,317,836	+		PP_4680	0.11	-3.0
5,317,836	+		PP_4680	0.11	-1.5
5,317,837	-		PP_4680	0.11	+0.8
5,317,837	-		PP_4680	0.11	-3.3
5,317,837	-		PP_4680	0.11	-1.3
5,317,855	+		PP_4680	0.12	-4.0
5,317,856	-		PP_4680	0.12	-1.3
5,317,878	+		PP_4680	0.13	-3.3
5,317,878	+		PP_4680	0.13	-1.5
5,317,879	-		PP_4680	0.13	-1.3
5,317,879	-		PP_4680	0.13	-1.2
5,317,902	+		PP_4680	0.15	-2.2
5,317,902	+		PP_4680	0.15	-2.0
5,317,903	-		PP_4680	0.15	-1.7
5,317,903	-		PP_4680	0.15	-1.3
5,317,977	+		PP_4680	0.19	-3.3