Strain Fitness in Pseudomonas putida KT2440 around PP_4662

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,288,291	-				+0.3
5,288,351	+				+1.0
5,288,351	+				+0.4
5,288,355	+				+0.3
5,288,355	+				-1.2
5,288,390	+				-1.2
5,288,390	+				-0.1
5,288,391	-				-1.7
5,288,391	-				-0.2
5,288,427	-				+1.4
5,288,427	-				-0.2
5,288,451	+				+0.5
5,288,451	+				+0.1
5,288,451	+				-1.5
5,288,452	-				-0.2
5,288,452	-				-0.8
5,288,454	+				+0.8
5,288,498	+				-1.7
5,288,498	+				-0.1
5,288,499	-				+1.0
5,288,543	+				-0.5
5,288,543	+				-0.9
5,288,543	+				-0.4
5,288,543	+				-0.0
5,288,544	-				+0.8
5,288,544	-				+0.5
5,288,544	-				-0.6
5,288,544	-				-0.3
5,288,544	-				-1.1
5,288,651	+		PP_4661	0.22	+0.9
5,288,651	+		PP_4661	0.22	-0.3
5,288,652	-		PP_4661	0.22	-0.6
5,288,738	+		PP_4661	0.35	+1.2
5,288,738	+		PP_4661	0.35	-0.8
5,288,738	+		PP_4661	0.35	-0.4
5,288,738	+		PP_4661	0.35	+0.4
5,288,738	+		PP_4661	0.35	-1.4
5,288,739	-		PP_4661	0.35	-0.4
5,288,739	-		PP_4661	0.35	-0.4
5,288,739	-		PP_4661	0.35	-1.5
5,288,739	-				+0.1
5,288,739	-		PP_4661	0.35	-0.3
5,288,739	-		PP_4661	0.35	-1.6
5,288,772	-		PP_4661	0.40	-0.8
5,288,796	+		PP_4661	0.44	+0.0
5,288,813	+		PP_4661	0.46	+0.3
5,288,814	-		PP_4661	0.46	+0.0
5,288,814	-		PP_4661	0.46	-0.6
5,288,814	-		PP_4661	0.46	+0.4
5,288,853	-		PP_4661	0.52	-0.6
5,288,853	-		PP_4661	0.52	-0.5
5,288,853	-		PP_4661	0.52	-0.3
5,288,900	+		PP_4661	0.59	-0.0
5,288,900	+		PP_4661	0.59	+0.3
5,288,901	-		PP_4661	0.59	+0.5
5,288,901	-		PP_4661	0.59	+0.9
5,288,951	+		PP_4661	0.67	-0.6
5,288,952	-		PP_4661	0.67	-1.2
5,289,063	+		PP_4661	0.83	-0.2
5,289,136	-				+0.5
5,289,136	-				-2.5
5,289,156	+				+0.7
5,289,178	-				+0.0
5,289,208	-				+0.3
5,289,208	-				+1.9
5,289,248	+				+0.3
5,289,277	+				-0.0
5,289,278	-				+0.8
5,289,278	-				-0.4
5,289,278	-				-1.5
5,289,338	+		PP_4662	0.26	-0.7
5,289,339	-		PP_4662	0.26	-0.9
5,289,506	+		PP_4662	0.79	-1.3
5,289,507	-		PP_4662	0.79	-1.0
5,289,507	-		PP_4662	0.79	-0.9
5,289,507	-		PP_4662	0.79	+0.2
5,289,748	-		PP_4663	0.24	+0.3
5,289,774	-		PP_4663	0.28	-0.4
5,289,833	-		PP_4663	0.36	-0.8
5,289,930	+		PP_4663	0.48	+2.4
5,289,930	+		PP_4663	0.48	-0.2
5,289,931	-		PP_4663	0.48	-0.4
5,289,958	+		PP_4663	0.52	-2.6
5,289,958	+		PP_4663	0.52	-1.8
5,289,959	-		PP_4663	0.52	-0.6
5,289,959	-		PP_4663	0.52	+0.3
5,289,959	-		PP_4663	0.52	+0.7
5,289,968	-		PP_4663	0.53	-1.2
5,290,266	+				+0.7
5,290,266	+				-0.5
5,290,266	+				-0.0
5,290,266	+				+0.3
5,290,266	+				-0.0
5,290,303	+				-1.4
5,290,304	-				+0.3
5,290,304	-				-0.3
5,290,368	+				+0.2
5,290,368	+				-2.1
5,290,369	-				+0.2
5,290,370	+				+0.6
5,290,370	+				+0.2
5,290,371	-				+1.9