Strain Fitness in Pseudomonas putida KT2440 around PP_4613

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,234,145	+		PP_4612	0.54	+0.3
5,234,145	+		PP_4612	0.54	+1.1
5,234,145	+		PP_4612	0.54	-1.0
5,234,145	+				+1.4
5,234,145	+		PP_4612	0.54	+3.1
5,234,145	+		PP_4612	0.54	+0.1
5,234,146	-		PP_4612	0.54	+0.8
5,234,149	+		PP_4612	0.54	-2.7
5,234,149	+		PP_4612	0.54	+0.4
5,234,149	+		PP_4612	0.54	-0.2
5,234,150	-		PP_4612	0.54	+2.4
5,234,163	+		PP_4612	0.56	-1.2
5,234,163	+		PP_4612	0.56	-0.8
5,234,163	+		PP_4612	0.56	+1.4
5,234,163	+		PP_4612	0.56	+0.6
5,234,163	+		PP_4612	0.56	+0.8
5,234,163	+		PP_4612	0.56	+1.0
5,234,163	+		PP_4612	0.56	-1.0
5,234,163	+		PP_4612	0.56	+0.6
5,234,163	+		PP_4612	0.56	-1.6
5,234,164	-		PP_4612	0.56	+0.9
5,234,164	-		PP_4612	0.56	+1.1
5,234,164	-		PP_4612	0.56	-0.4
5,234,164	-		PP_4612	0.56	+1.9
5,234,164	-		PP_4612	0.56	-1.7
5,234,164	-		PP_4612	0.56	+1.1
5,234,168	+		PP_4612	0.56	-0.2
5,234,168	+		PP_4612	0.56	-0.0
5,234,169	-		PP_4612	0.56	-0.3
5,234,169	-		PP_4612	0.56	+1.7
5,234,169	-		PP_4612	0.56	-0.1
5,234,193	+		PP_4612	0.59	-1.2
5,234,210	+		PP_4612	0.61	+0.7
5,234,211	-		PP_4612	0.61	+0.8
5,234,211	-		PP_4612	0.61	+0.1
5,234,212	+		PP_4612	0.61	+1.4
5,234,213	-		PP_4612	0.61	+1.4
5,234,463	-		PP_4612	0.88	+0.0
5,234,571	-				-0.7
5,234,571	-				+0.8
5,234,760	+				+0.4
5,234,760	+				+0.2
5,234,760	+				+1.4
5,234,760	+				-2.9
5,234,760	+				-0.3
5,234,760	+				+0.7
5,234,761	-				-2.0
5,234,761	-				-1.2
5,235,061	+		PP_4613	0.17	+0.4
5,235,061	+		PP_4613	0.17	+0.1
5,235,062	-		PP_4613	0.17	-0.4
5,235,062	-		PP_4613	0.17	+0.8
5,235,089	-		PP_4613	0.18	-0.9
5,235,122	+		PP_4613	0.20	+0.7
5,235,123	-		PP_4613	0.20	+0.2
5,235,123	-		PP_4613	0.20	+1.4
5,235,123	-		PP_4613	0.20	+0.7
5,235,291	+				+0.8
5,235,378	+		PP_4613	0.31	-0.4
5,235,561	+		PP_4613	0.38	+1.2
5,235,678	+		PP_4613	0.43	+0.1
5,235,678	+		PP_4613	0.43	-0.5
5,235,679	-		PP_4613	0.44	+0.7
5,235,679	-		PP_4613	0.44	+0.0
5,235,681	+		PP_4613	0.44	+1.1
5,235,681	+		PP_4613	0.44	-1.5
5,235,681	+		PP_4613	0.44	-0.5
5,235,682	-		PP_4613	0.44	-1.0
5,235,682	-		PP_4613	0.44	+0.2
5,235,682	-		PP_4613	0.44	-1.5
5,235,682	-		PP_4613	0.44	-1.0
5,235,807	+		PP_4613	0.49	+0.1
5,235,870	+		PP_4613	0.52	-0.7
5,235,912	+		PP_4613	0.54	+1.1
5,235,913	-		PP_4613	0.54	+0.9
5,235,913	-		PP_4613	0.54	-1.0
5,236,227	+		PP_4613	0.67	+0.5
5,236,227	+		PP_4613	0.67	+2.0
5,236,227	+		PP_4613	0.67	-0.2
5,236,227	+		PP_4613	0.67	+1.6
5,236,227	+		PP_4613	0.67	+0.6
5,236,227	+		PP_4613	0.67	+2.4
5,236,228	-		PP_4613	0.67	+0.8
5,236,260	+		PP_4613	0.69	+1.5
5,236,260	+		PP_4613	0.69	+1.4
5,236,260	+		PP_4613	0.69	+0.5
5,236,260	+		PP_4613	0.69	+1.0
5,236,260	+		PP_4613	0.69	-1.3
5,236,260	+		PP_4613	0.69	+0.8
5,236,261	-		PP_4613	0.69	+0.1
5,236,261	-		PP_4613	0.69	+0.1
5,236,261	-		PP_4613	0.69	+0.5
5,236,261	-		PP_4613	0.69	-1.0
5,236,261	-		PP_4613	0.69	+1.1
5,236,323	+		PP_4613	0.71	+0.2
5,236,539	+		PP_4613	0.81	+0.0
5,236,540	-		PP_4613	0.81	+0.3
5,236,593	+		PP_4613	0.83	+0.0
5,236,593	+		PP_4613	0.83	-0.3
5,236,593	+		PP_4613	0.83	+2.2
5,236,594	-		PP_4613	0.83	+0.8
5,236,594	-		PP_4613	0.83	-0.2
5,236,594	-		PP_4613	0.83	+1.6
5,236,743	+		PP_4613	0.89	+0.5
5,236,743	+		PP_4613	0.89	-0.3
5,236,806	+				-0.2
5,236,807	-				-2.0
5,236,807	-				+1.5
5,236,807	-				+0.1
5,236,902	+				-1.3
5,236,905	+				-0.2
5,236,905	+				+1.2
5,236,905	+				-0.6
5,236,906	-				+0.5
5,236,918	-				+1.4
5,236,918	-				+1.4
5,236,918	-				+1.1
5,236,918	-				+1.1
5,236,941	+				-0.1
5,236,941	+				-0.6
5,236,941	+				-0.5
5,236,942	-				+0.8
5,236,942	-				-0.4
5,236,958	+				-1.0
5,236,958	+				-0.6
5,236,958	+				-1.2
5,236,958	+				-1.3
5,236,959	-				+0.1
5,236,959	-				-0.7
5,236,959	-				-0.5
5,236,965	+				+0.4
5,236,966	-				-1.4
5,237,017	+				+1.4
5,237,018	-				-0.2
5,237,088	-				-0.2
5,237,932	+				-0.5
5,237,932	+				+0.1
5,237,933	-				+0.8