Strain Fitness in Pseudomonas putida KT2440 around PP_4508

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,120,780	+		PP_4507	0.29	+0.7
5,120,922	+		PP_4507	0.39	-0.6
5,120,923	-		PP_4507	0.39	+0.2
5,120,938	+		PP_4507	0.40	-0.8
5,121,009	-		PP_4507	0.45	+3.4
5,121,085	+		PP_4507	0.50	-1.4
5,121,086	-		PP_4507	0.50	+0.8
5,121,086	-		PP_4507	0.50	+2.4
5,121,086	-		PP_4507	0.50	-0.6
5,121,086	-		PP_4507	0.50	-0.4
5,121,123	+		PP_4507	0.53	+2.4
5,121,124	-		PP_4507	0.53	+0.1
5,121,127	+		PP_4507	0.53	-0.6
5,121,128	-		PP_4507	0.53	-1.3
5,121,228	+		PP_4507	0.60	+0.4
5,121,228	+		PP_4507	0.60	+0.8
5,121,234	+		PP_4507	0.60	-0.2
5,121,234	+		PP_4507	0.60	+1.4
5,121,235	-		PP_4507	0.60	-0.9
5,121,235	-		PP_4507	0.60	+1.8
5,121,235	-		PP_4507	0.60	+1.4
5,121,327	+		PP_4507	0.67	+0.4
5,121,342	-		PP_4507	0.68	+1.4
5,121,438	+		PP_4507	0.74	-1.4
5,121,438	+		PP_4507	0.74	+0.1
5,121,438	+		PP_4507	0.74	-1.5
5,121,438	+		PP_4507	0.74	+2.4
5,121,438	+		PP_4507	0.74	+1.4
5,121,438	+		PP_4507	0.74	-0.0
5,121,438	+		PP_4507	0.74	-0.4
5,121,438	+		PP_4507	0.74	-0.2
5,121,438	+		PP_4507	0.74	+2.4
5,121,439	-		PP_4507	0.74	-1.0
5,121,439	-		PP_4507	0.74	+1.8
5,121,439	-		PP_4507	0.74	-0.4
5,121,439	-		PP_4507	0.74	+1.2
5,121,439	-		PP_4507	0.74	-0.4
5,121,439	-		PP_4507	0.74	+2.1
5,121,439	-		PP_4507	0.74	+0.1
5,121,439	-		PP_4507	0.74	-0.4
5,121,439	-		PP_4507	0.74	-0.6
5,121,659	-		PP_4507	0.89	-1.6
5,121,845	+				-0.2
5,121,920	+		PP_4508	0.15	-0.5
5,121,921	-		PP_4508	0.15	-0.1
5,121,959	+		PP_4508	0.19	-1.4
5,121,960	-		PP_4508	0.19	-0.6
5,121,960	-		PP_4508	0.19	-0.6
5,121,960	-		PP_4508	0.19	+0.3
5,121,960	-		PP_4508	0.19	-1.8
5,122,301	+		PP_4508	0.54	-0.4
5,122,301	+		PP_4508	0.54	-0.6
5,122,301	+		PP_4508	0.54	-0.4
5,122,302	-		PP_4508	0.54	-0.5
5,122,302	-		PP_4508	0.54	-1.4
5,122,302	-		PP_4508	0.54	-0.5
5,122,466	+		PP_4508	0.71	-2.8
5,122,467	-		PP_4508	0.71	+0.3
5,122,524	-		PP_4508	0.77	-0.2
5,122,577	+		PP_4508	0.82	-0.2
5,122,844	+				-2.2
5,122,845	-				-0.2
5,122,875	+				+0.1
5,122,875	+				-1.0
5,122,876	-				+1.0
5,122,890	+				+0.9
5,122,890	+				+0.1
5,122,890	+				+0.5
5,122,902	+				+3.4
5,122,902	+				-0.5
5,122,902	+				+1.2
5,122,921	+				-0.6
5,122,922	-				-0.2
5,122,922	-				-0.5
5,122,948	+				-0.1
5,122,948	+				-0.2
5,122,949	-				+0.2
5,122,964	-				-0.6
5,122,964	-				-1.2
5,122,966	+				+0.0
5,123,023	+		PP_4509	0.12	+1.2
5,123,023	+		PP_4509	0.12	-0.9
5,123,029	+		PP_4509	0.14	+0.5
5,123,030	-		PP_4509	0.15	+0.3
5,123,032	+		PP_4509	0.15	-1.2
5,123,033	-		PP_4509	0.16	+0.5
5,123,096	+		PP_4509	0.36	+2.4
5,123,097	-		PP_4509	0.36	-0.6
5,123,110	+		PP_4509	0.40	-1.3
5,123,111	-		PP_4509	0.41	-0.5
5,123,220	+		PP_4509	0.76	+0.6
5,123,220	+		PP_4509	0.76	-0.3
5,123,233	+		PP_4509	0.80	+0.2
5,123,233	+		PP_4509	0.80	-0.4
5,123,277	-				+0.8
5,123,293	+				-0.6
5,123,293	+				+0.5
5,123,294	-				-1.0
5,123,335	+				+1.2
5,123,384	-				+0.5
5,123,495	+		PP_4510	0.20	+0.7
5,123,495	+		PP_4510	0.20	-2.2
5,123,496	-		PP_4510	0.20	-0.2
5,123,496	-		PP_4510	0.20	+0.3
5,123,496	-		PP_4510	0.20	+0.2
5,123,602	+		PP_4510	0.34	-1.0
5,123,621	-		PP_4510	0.36	-0.5
5,123,626	+		PP_4510	0.37	+0.9
5,123,626	+		PP_4510	0.37	+1.1
5,123,626	+		PP_4510	0.37	-0.7
5,123,684	-		PP_4510	0.44	-1.6
5,123,684	-		PP_4510	0.44	-0.6