Strain Fitness in Pseudomonas putida KT2440 around PP_4505

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
5,117,259	-		PP_4503	0.51	+2.4
5,117,318	-		PP_4503	0.60	-0.7
5,117,473	+		PP_4503	0.83	+0.6
5,117,474	-		PP_4503	0.83	-0.8
5,117,549	+				+1.4
5,117,550	-				+0.5
5,117,550	-				+0.3
5,117,572	+				+0.5
5,117,573	-				-0.4
5,117,575	+				+1.1
5,117,575	+				-2.4
5,117,576	-				+0.4
5,117,576	-				+0.1
5,117,576	-				+0.4
5,117,576	-				+0.1
5,117,576	-				-1.1
5,117,576	-				-0.3
5,117,578	+				-0.5
5,117,579	-				+0.4
5,117,579	-				-0.9
5,117,587	+				-0.2
5,117,587	+				-0.2
5,117,658	+				+0.2
5,117,658	+				+1.2
5,117,659	-				-0.9
5,117,669	+				+0.7
5,117,670	-				+0.4
5,117,960	-		PP_4504	0.50	-1.9
5,117,960	-		PP_4504	0.50	-1.0
5,118,166	+				-0.3
5,118,166	+				-0.4
5,118,167	-				+0.3
5,118,167	-				-0.2
5,118,167	-				+0.2
5,118,221	+				-0.6
5,118,222	-				-0.9
5,118,393	-		PP_4505	0.12	-2.5
5,118,587	+		PP_4505	0.27	-1.3
5,118,614	+		PP_4505	0.29	-0.8
5,118,614	+		PP_4505	0.29	-0.9
5,118,626	+		PP_4505	0.30	-0.7
5,118,627	-		PP_4505	0.30	-0.4
5,118,627	-		PP_4505	0.30	-1.3
5,118,803	+		PP_4505	0.43	-0.4
5,118,803	+		PP_4505	0.43	-2.4
5,118,803	+		PP_4505	0.43	+0.4
5,118,803	+		PP_4505	0.43	-1.7
5,118,803	+		PP_4505	0.43	-2.0
5,118,804	-		PP_4505	0.43	-2.5
5,118,925	-		PP_4505	0.52	-2.0
5,118,925	-		PP_4505	0.52	-3.0
5,119,612	+				-1.2
5,119,699	+				+0.1
5,119,699	+				+1.4
5,119,699	+				+0.3
5,119,700	-				-0.9
5,119,700	-				-0.4
5,119,712	+				-1.3
5,119,712	+				-0.1
5,119,712	+				-1.2
5,119,712	+				-0.4
5,119,713	-				+0.3
5,119,713	-				-1.1
5,119,713	-				+0.2
5,119,715	+				-0.7
5,119,715	+				-1.6
5,119,777	+		PP_4506	0.14	-1.4
5,119,777	+		PP_4506	0.14	-1.8
5,119,777	+		PP_4506	0.14	-0.4
5,119,777	+		PP_4506	0.14	+0.3
5,119,777	+		PP_4506	0.14	+0.8
5,119,778	-		PP_4506	0.14	-1.1
5,119,778	-		PP_4506	0.14	-0.6
5,119,778	-		PP_4506	0.14	-0.8
5,119,778	-		PP_4506	0.14	+0.1
5,119,778	-		PP_4506	0.14	-1.4
5,119,784	-		PP_4506	0.15	-1.9
5,119,784	-		PP_4506	0.15	-0.6
5,119,857	-		PP_4506	0.28	+1.7
5,119,857	-		PP_4506	0.28	-1.0
5,120,111	+		PP_4506	0.74	-0.2
5,120,112	-		PP_4506	0.74	+0.2
5,120,292	+				+1.1
5,120,293	-				-0.3
5,120,299	+				-0.2
5,120,300	-				+0.4
5,120,300	-				+0.7
5,120,300	-				-1.7
5,120,300	-				+0.7
5,120,300	-				+2.4
5,120,300	-				+0.4
5,120,315	+				+0.2
5,120,315	+				+0.6
5,120,315	+				-0.0
5,120,316	-				+0.1
5,120,316	-				-0.7
5,120,316	-				+0.1
5,120,319	+				+0.3
5,120,319	+				-0.3
5,120,319	+				-1.7
5,120,319	+				-0.3
5,120,320	-				-1.7
5,120,320	-				-0.6
5,120,320	-				+0.3
5,120,374	+				+2.4
5,120,374	+				+0.4
5,120,375	-				-0.7
5,120,412	-				-0.8
5,120,554	+		PP_4507	0.13	-1.6
5,120,554	+		PP_4507	0.13	-0.3
5,120,555	-		PP_4507	0.14	+0.1
5,120,555	-		PP_4507	0.14	+0.1
5,120,555	-		PP_4507	0.14	+1.4
5,120,555	-		PP_4507	0.14	+0.4
5,120,555	-		PP_4507	0.14	+0.1