Strain Fitness in Pseudomonas putida KT2440 around PP_4385

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,977,791	-				-0.4
4,977,791	-				-0.3
4,977,791	-				+1.2
4,977,791	-				+0.1
4,977,902	-				+0.9
4,977,986	+		PP_4384	0.12	-1.3
4,977,986	+		PP_4384	0.12	+0.9
4,977,986	+		PP_4384	0.12	-0.1
4,977,986	+		PP_4384	0.12	-1.0
4,977,987	-		PP_4384	0.12	+0.9
4,977,987	-		PP_4384	0.12	+0.7
4,977,987	-		PP_4384	0.12	+0.1
4,977,987	-		PP_4384	0.12	-0.4
4,978,141	+		PP_4384	0.34	-1.5
4,978,228	-		PP_4384	0.47	-2.8
4,978,230	-		PP_4384	0.47	-1.5
4,978,269	+		PP_4384	0.52	-0.0
4,978,269	+		PP_4384	0.52	+1.2
4,978,269	+		PP_4384	0.52	+0.4
4,978,269	+		PP_4384	0.52	-1.8
4,978,270	-		PP_4384	0.53	-0.2
4,978,424	+		PP_4384	0.75	-0.8
4,978,425	-		PP_4384	0.75	+0.4
4,978,452	-		PP_4384	0.79	-0.0
4,978,452	-		PP_4384	0.79	-1.8
4,978,452	-		PP_4384	0.79	+0.3
4,978,452	-		PP_4384	0.79	+0.0
4,978,508	+		PP_4384	0.87	-1.5
4,978,508	+		PP_4384	0.87	-0.1
4,978,509	-		PP_4384	0.87	-1.4
4,978,512	-		PP_4384	0.87	+0.1
4,978,606	+				-1.0
4,978,606	+				-0.1
4,978,606	+				-0.2
4,978,607	-				-0.2
4,978,607	-				+0.2
4,978,607	-				+0.9
4,978,637	+				-0.5
4,978,637	+				-0.8
4,978,637	+				-0.6
4,978,637	+				-1.5
4,978,637	+				-0.5
4,978,637	+				-0.4
4,978,638	-				+0.5
4,978,651	-				-0.3
4,978,703	+				-0.8
4,978,993	-		PP_4385	0.45	+0.2
4,979,021	+		PP_4385	0.49	-0.4
4,979,080	-		PP_4385	0.56	-2.5
4,979,081	+		PP_4385	0.57	-0.5
4,979,082	-		PP_4385	0.57	-1.7
4,979,082	-		PP_4385	0.57	-1.9
4,979,286	-		PP_4385	0.83	+0.1
4,979,286	-		PP_4385	0.83	-1.3
4,979,448	-				+0.4
4,979,519	+				+0.1
4,979,914	+		PP_4386	0.60	-0.8
4,979,914	+		PP_4386	0.60	+0.6
4,979,914	+		PP_4386	0.60	+0.4
4,979,914	+		PP_4386	0.60	+1.4
4,979,914	+		PP_4386	0.60	-0.4
4,979,914	+		PP_4386	0.60	-0.1
4,979,915	-		PP_4386	0.60	-1.6
4,979,915	-		PP_4386	0.60	-2.9
4,979,915	-		PP_4386	0.60	-0.1
4,980,193	+				-1.0
4,980,193	+				-1.3
4,980,194	-				-0.9
4,980,227	+				-0.6
4,980,321	+				+0.4
4,980,398	+		PP_4387	0.15	+0.2
4,980,398	+		PP_4387	0.15	+0.1