Strain Fitness in Pseudomonas putida KT2440 around PP_4213

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,759,410	-		PP_4212	0.77	-0.4
4,759,642	+				-0.2
4,759,713	+				+0.6
4,759,713	+				-0.3
4,759,714	-				-1.0
4,759,714	-				+0.3
4,759,714	-				+0.3
4,759,790	-				-0.6
4,759,790	-				+0.2
4,759,815	+				-0.5
4,759,816	-				+0.6
4,759,835	+				+0.1
4,759,836	-				+0.4
4,759,934	+				-0.1
4,760,014	+				+1.4
4,760,014	+				-2.9
4,760,014	+				-1.7
4,760,014	+				-0.6
4,760,065	+				+0.4
4,760,416	+		PP_4213	0.34	-0.7
4,760,488	+		PP_4213	0.40	-1.0
4,760,488	+		PP_4213	0.40	-1.4
4,760,488	+		PP_4213	0.40	+2.4
4,760,875	+		PP_4213	0.67	-1.4
4,761,104	+		PP_4213	0.84	-1.4
4,761,104	+		PP_4213	0.84	+1.1
4,761,240	+				-0.3
4,761,732	+		PP_4214	0.30	-1.3
4,761,789	+		PP_4214	0.35	+1.4
4,761,789	+		PP_4214	0.35	-0.2
4,761,813	+		PP_4214	0.37	+0.5
4,761,850	+		PP_4214	0.39	-0.9
4,761,851	-		PP_4214	0.40	+2.4
4,761,892	+		PP_4214	0.43	+0.8
4,761,895	+		PP_4214	0.43	+2.4
4,762,263	+		PP_4214	0.72	+0.3