Strain Fitness in Pseudomonas putida KT2440 around PP_4208

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,752,206	+				+0.1
4,752,213	+				-0.1
4,752,213	+				+0.1
4,752,214	-				+2.0
4,752,214	-				+0.3
4,752,256	+				-0.5
4,752,262	-				-0.7
4,752,262	-				-2.0
4,752,297	+		PP_4207	0.11	+0.1
4,752,297	+		PP_4207	0.11	+0.1
4,752,325	+		PP_4207	0.17	-0.9
4,752,468	-		PP_4207	0.47	+0.3
4,752,546	+		PP_4207	0.63	-0.3
4,752,636	-		PP_4207	0.82	+0.4
4,752,746	-				+3.4
4,752,768	-				+0.7
4,752,768	-				-0.1
4,752,768	-				+1.4
4,752,786	-				+0.2
4,752,822	+				-0.5
4,752,823	-				-0.1
4,752,865	-				+0.7
4,753,122	+		PP_4208	0.48	-2.3
4,753,122	+		PP_4208	0.48	-2.8
4,753,123	-		PP_4208	0.48	-0.1
4,753,123	-		PP_4208	0.48	-0.4
4,753,123	-		PP_4208	0.48	-2.8
4,753,123	-		PP_4208	0.48	-1.7
4,753,123	-		PP_4208	0.48	-1.4
4,753,220	+		PP_4208	0.69	-0.6
4,753,280	+		PP_4208	0.81	-3.0
4,753,280	+		PP_4208	0.81	-0.6
4,753,280	+		PP_4208	0.81	-1.6
4,753,280	+		PP_4208	0.81	-2.6
4,753,281	-		PP_4208	0.82	-2.6
4,753,281	-		PP_4208	0.82	-2.8
4,753,317	+		PP_4208	0.89	-0.2
4,753,355	+				-0.8
4,753,355	+				-3.6
4,753,356	-				-2.0
4,753,356	-				-2.9
4,753,356	-				+2.4
4,753,358	+				-3.4
4,753,358	+				-2.3
4,753,358	+				-1.6
4,753,359	-				-2.2
4,753,359	-				-2.8
4,753,359	-				-2.5
4,753,359	-				-1.1
4,753,375	-				-2.6
4,753,425	+				-1.8
4,753,426	-				-0.7
4,753,481	+				-3.4
4,753,481	+				-1.6
4,753,482	-				+1.8
4,753,515	+				-1.3
4,753,516	-				+0.5
4,753,733	-		PP_4209	0.13	+0.5
4,753,733	-		PP_4209	0.13	-1.3
4,753,769	-		PP_4209	0.16	-0.9
4,753,911	-		PP_4209	0.28	-0.5
4,754,070	+		PP_4209	0.41	-0.7
4,754,070	+		PP_4209	0.41	+0.2
4,754,071	-		PP_4209	0.42	-0.5
4,754,130	+		PP_4209	0.47	-0.5
4,754,150	+		PP_4209	0.48	-2.0
4,754,151	-		PP_4209	0.48	-0.0
4,754,165	-		PP_4209	0.50	+0.1
4,754,165	-		PP_4209	0.50	+1.0
4,754,165	-		PP_4209	0.50	-1.0