Strain Fitness in Pseudomonas putida KT2440 around PP_3999

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,506,452	+		PP_3998	0.15	+0.6
4,506,452	+		PP_3998	0.15	+0.1
4,506,452	+		PP_3998	0.15	+0.1
4,506,452	+		PP_3998	0.15	-0.4
4,506,452	+		PP_3998	0.15	+0.8
4,506,452	+		PP_3998	0.15	-0.5
4,506,452	+		PP_3998	0.15	-0.6
4,506,452	+		PP_3998	0.15	+0.2
4,506,452	+		PP_3998	0.15	-0.8
4,506,453	-		PP_3998	0.15	+0.3
4,506,453	-		PP_3998	0.15	+0.1
4,506,453	-		PP_3998	0.15	+1.1
4,506,453	-		PP_3998	0.15	+0.3
4,506,453	-		PP_3998	0.15	-1.0
4,506,453	-		PP_3998	0.15	-0.1
4,506,453	-		PP_3998	0.15	-1.0
4,506,453	-		PP_3998	0.15	+0.2
4,506,453	-		PP_3998	0.15	+0.8
4,506,453	-		PP_3998	0.15	-0.6
4,506,620	+		PP_3998	0.32	+0.8
4,506,620	+		PP_3998	0.32	+0.1
4,506,621	-		PP_3998	0.32	-0.3
4,506,635	+		PP_3998	0.34	+0.1
4,506,635	+		PP_3998	0.34	-0.1
4,506,636	-		PP_3998	0.34	+2.5
4,506,636	-		PP_3998	0.34	+0.2
4,506,636	-		PP_3998	0.34	-0.3
4,506,656	+		PP_3998	0.36	+1.2
4,506,657	-		PP_3998	0.36	-0.5
4,506,657	-		PP_3998	0.36	+0.4
4,506,681	+		PP_3998	0.38	-0.4
4,506,682	-		PP_3998	0.39	-0.6
4,506,682	-		PP_3998	0.39	-1.0
4,506,682	-		PP_3998	0.39	-0.8
4,506,741	+		PP_3998	0.45	-1.2
4,506,775	+				+1.7
4,506,775	+		PP_3998	0.48	+1.5
4,506,789	-		PP_3998	0.49	-0.6
4,506,836	+		PP_3998	0.54	-0.8
4,506,836	+		PP_3998	0.54	-0.1
4,506,837	-		PP_3998	0.54	+0.1
4,506,861	-		PP_3998	0.57	-0.3
4,506,877	+		PP_3998	0.58	-0.0
4,506,878	-		PP_3998	0.59	-1.0
4,506,878	-		PP_3998	0.59	+0.7
4,506,894	-		PP_3998	0.60	+0.1
4,506,932	+		PP_3998	0.64	-0.5
4,506,933	-		PP_3998	0.64	-1.1
4,506,933	-		PP_3998	0.64	-0.5
4,506,948	+		PP_3998	0.66	-0.6
4,506,949	-		PP_3998	0.66	+0.9
4,506,962	+		PP_3998	0.67	+0.1
4,506,963	-		PP_3998	0.67	-0.8
4,506,977	+		PP_3998	0.69	+0.7
4,506,996	+		PP_3998	0.71	+1.6
4,506,996	+		PP_3998	0.71	-1.6
4,506,997	-		PP_3998	0.71	+1.5
4,506,997	-		PP_3998	0.71	-0.7
4,507,010	-		PP_3998	0.72	+2.5
4,507,076	+		PP_3998	0.79	-0.9
4,507,110	-		PP_3998	0.82	-0.7
4,507,163	+		PP_3998	0.88	-0.6
4,507,166	+		PP_3998	0.88	+0.8
4,507,166	+		PP_3998	0.88	+0.3
4,507,166	+		PP_3998	0.88	-0.4
4,507,166	+		PP_3998	0.88	+0.9
4,507,166	+		PP_3998	0.88	-0.3
4,507,166	+		PP_3998	0.88	-0.4
4,507,167	-		PP_3998	0.88	+0.4
4,507,167	-		PP_3998	0.88	+0.2
4,507,167	-		PP_3998	0.88	-1.0
4,507,329	+				-0.6
4,507,330	-				-0.0
4,507,356	+				-0.8
4,507,356	+				+0.2
4,507,357	-				-0.7
4,507,357	-				+0.2
4,507,357	-				+3.1
4,507,571	+		PP_3999	0.13	-0.8
4,507,636	+		PP_3999	0.18	-0.1
4,507,636	+		PP_3999	0.18	+0.2
4,507,636	+		PP_3999	0.18	+0.9
4,507,636	+		PP_3999	0.18	-0.5
4,507,637	-		PP_3999	0.18	-0.1
4,507,637	-		PP_3999	0.18	+0.5
4,507,735	+		PP_3999	0.25	-1.5
4,507,736	-		PP_3999	0.25	+0.9
4,507,766	-		PP_3999	0.27	+0.9
4,507,977	+		PP_3999	0.42	-0.1
4,507,977	+		PP_3999	0.42	-0.8
4,507,977	+		PP_3999	0.42	-1.5
4,507,978	-		PP_3999	0.42	-1.1
4,507,981	+		PP_3999	0.43	-1.1
4,507,981	+		PP_3999	0.43	-0.4
4,507,981	+		PP_3999	0.43	+2.5
4,507,981	+		PP_3999	0.43	-1.1
4,507,981	+		PP_3999	0.43	+0.2
4,507,982	-		PP_3999	0.43	-0.5
4,507,982	-		PP_3999	0.43	-1.6
4,507,982	-		PP_3999	0.43	+0.7
4,508,009	+		PP_3999	0.45	+0.9
4,508,009	+		PP_3999	0.45	-0.1
4,508,009	+		PP_3999	0.45	-1.1
4,508,009	+		PP_3999	0.45	-0.7
4,508,009	+		PP_3999	0.45	+1.5
4,508,010	-		PP_3999	0.45	+0.9
4,508,010	-		PP_3999	0.45	-1.1
4,508,010	-		PP_3999	0.45	+0.5
4,508,010	-		PP_3999	0.45	+0.9
4,508,042	-		PP_3999	0.47	+0.9
4,508,125	+		PP_3999	0.53	-0.8
4,508,126	-		PP_3999	0.53	+0.3
4,508,276	-		PP_3999	0.64	-0.1
4,508,329	+		PP_3999	0.68	+2.5
4,508,413	-		PP_3999	0.74	+0.3
4,508,453	+		PP_3999	0.76	-0.8
4,508,454	-		PP_3999	0.77	+0.5
4,508,454	-		PP_3999	0.77	-0.3
4,508,454	-		PP_3999	0.77	-0.8
4,508,587	+		PP_3999	0.86	-1.3
4,508,774	-				+0.9
4,508,774	-				-0.7