Strain Fitness in Pseudomonas putida KT2440 around PP_3833

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,357,265	+		PP_3831	0.62	+1.0
4,357,292	-		PP_3831	0.65	-1.4
4,357,349	-		PP_3831	0.70	+0.1
4,357,361	+		PP_3831	0.71	+2.6
4,357,361	+		PP_3831	0.71	+1.9
4,357,362	-		PP_3831	0.71	-0.5
4,357,401	+		PP_3831	0.75	+1.8
4,357,402	-		PP_3831	0.75	+1.5
4,357,455	+				-1.0
4,357,487	-		PP_3831	0.84	-0.9
4,357,511	+		PP_3831	0.86	+2.2
4,357,511	+		PP_3831	0.86	+2.9
4,357,511	+		PP_3831	0.86	+3.6
4,357,512	-		PP_3831	0.86	-0.1
4,357,512	-		PP_3831	0.86	-0.9
4,357,538	+		PP_3831	0.89	+2.9
4,357,538	+		PP_3831	0.89	+2.8
4,357,538	+		PP_3831	0.89	+2.6
4,357,538	+		PP_3831	0.89	+3.2
4,357,539	-		PP_3831	0.89	+0.4
4,357,539	-		PP_3831	0.89	-0.3
4,357,710	+				+2.1
4,357,807	+				+4.6
4,357,807	+				+4.4
4,357,807	+				+4.3
4,357,808	-				-1.6
4,357,810	+				+3.5
4,357,871	+				+0.8
4,357,885	+				+0.5
4,357,885	+				+2.5
4,357,885	+				-1.1
4,357,885	+				+1.3
4,357,886	-				-0.8
4,357,886	-				+0.5
4,357,886	-				-2.1
4,357,886	-				-1.0
4,357,887	+				+3.3
4,357,887	+				-0.7
4,357,887	+				+0.8
4,357,887	+				+0.3
4,357,887	+				+0.9
4,357,887	+				-1.6
4,357,888	-				-0.4
4,357,888	-				-0.4
4,357,888	-				-0.5
4,357,888	-				-1.7
4,357,905	+				+0.1
4,357,905	+				+0.0
4,357,905	+				+3.5
4,357,906	-				-0.2
4,357,906	-				-0.8
4,357,906	-				-2.6
4,357,906	-				-0.6
4,357,906	-				-1.2
4,357,925	+				+0.9
4,358,154	+				-1.2
4,358,154	+				+3.1
4,358,154	+				+0.9
4,358,154	+				-0.8
4,358,157	+				+2.5
4,358,158	-				-0.7
4,358,208	+		PP_3833	0.18	-1.2
4,358,209	-		PP_3833	0.18	+0.3
4,358,209	-		PP_3833	0.18	-0.4
4,358,341	+		PP_3833	0.55	-1.8
4,358,341	+		PP_3833	0.55	-0.3
4,358,341	+		PP_3833	0.55	-1.9
4,358,342	-		PP_3833	0.56	-0.6
4,358,342	-		PP_3833	0.56	+0.9
4,358,467	+				-0.1
4,358,467	+				+0.2
4,358,468	-				-0.7
4,358,581	+				-0.5
4,358,581	+				-0.3
4,358,581	+				-1.7
4,358,582	-				-1.5
4,358,667	-		PP_3834	0.11	+1.5
4,358,667	-		PP_3834	0.11	-1.9
4,358,701	+		PP_3834	0.13	-1.7
4,358,701	+		PP_3834	0.13	+0.8
4,358,701	+		PP_3834	0.13	-0.2
4,358,701	+		PP_3834	0.13	-0.5
4,358,701	+		PP_3834	0.13	+0.2
4,358,702	-		PP_3834	0.13	+0.4
4,358,702	-		PP_3834	0.13	+1.0
4,358,702	-		PP_3834	0.13	-0.7
4,358,702	-		PP_3834	0.13	+1.0
4,358,702	-		PP_3834	0.13	-2.2
4,358,702	-		PP_3834	0.13	-0.4
4,358,857	+		PP_3834	0.24	-2.2
4,358,858	-		PP_3834	0.24	+0.3
4,358,992	+		PP_3834	0.33	-0.7
4,359,021	+		PP_3834	0.35	-0.6
4,359,066	+		PP_3834	0.38	-0.4
4,359,215	+		PP_3834	0.49	-0.0
4,359,247	+		PP_3834	0.51	-0.9
4,359,248	-		PP_3834	0.51	-1.3
4,359,291	+		PP_3834	0.54	+0.4
4,359,292	-		PP_3834	0.54	-0.8
4,359,482	+		PP_3834	0.67	-0.2
4,359,482	+		PP_3834	0.67	-0.5
4,359,482	+		PP_3834	0.67	-0.6
4,359,482	+		PP_3834	0.67	+0.8
4,359,483	-		PP_3834	0.67	-0.9