Strain Fitness in Pseudomonas putida KT2440 around PP_3828

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,353,167	-		PP_3827	0.27	-1.6
4,353,167	-		PP_3827	0.27	-0.7
4,353,169	+		PP_3827	0.27	-0.1
4,353,169	+		PP_3827	0.27	-0.1
4,353,169	+		PP_3827	0.27	-1.8
4,353,169	+		PP_3827	0.27	+0.8
4,353,170	-		PP_3827	0.27	-0.7
4,353,170	-		PP_3827	0.27	-2.1
4,353,321	-		PP_3827	0.41	-0.4
4,353,341	+		PP_3827	0.43	-1.6
4,353,342	-		PP_3827	0.43	-1.0
4,353,342	-		PP_3827	0.43	-1.2
4,353,418	+		PP_3827	0.50	-1.7
4,353,418	+		PP_3827	0.50	+0.5
4,353,474	-		PP_3827	0.55	-2.1
4,353,491	+		PP_3827	0.57	-0.2
4,353,491	+		PP_3827	0.57	-1.1
4,353,541	-		PP_3827	0.61	+0.2
4,353,610	+		PP_3827	0.68	+1.1
4,353,611	-		PP_3827	0.68	+0.2
4,353,809	+		PP_3827	0.86	-0.4
4,353,809	+		PP_3827	0.86	+0.3
4,353,809	+		PP_3827	0.86	-0.1
4,353,835	-		PP_3827	0.88	-1.9
4,353,835	-		PP_3827	0.88	-1.7
4,353,835	-		PP_3827	0.88	+1.5
4,353,842	-		PP_3827	0.89	+0.2
4,353,873	-				-0.8
4,353,924	+				+0.2
4,354,002	+				+0.8
4,354,004	+				-0.3
4,354,005	-				+1.0
4,354,008	+				+0.1
4,354,235	+		PP_3828	0.25	+0.1
4,354,235	+		PP_3828	0.25	+0.0
4,354,326	+		PP_3828	0.37	+0.9
4,354,326	+		PP_3828	0.37	+0.5
4,354,343	-		PP_3828	0.40	-0.1
4,354,346	+		PP_3828	0.40	+1.5
4,354,389	-		PP_3828	0.46	+0.2
4,354,392	+		PP_3828	0.46	+0.1
4,354,392	+		PP_3828	0.46	+0.4
4,354,393	-		PP_3828	0.46	-0.9
4,354,393	-		PP_3828	0.46	-0.1
4,354,393	-		PP_3828	0.46	-0.3
4,354,428	-		PP_3828	0.51	-1.6
4,354,428	-		PP_3828	0.51	+0.9
4,354,466	+		PP_3828	0.56	-2.0
4,354,488	+		PP_3828	0.59	-0.7
4,354,488	+		PP_3828	0.59	+0.3
4,354,488	+		PP_3828	0.59	-1.5
4,354,489	-		PP_3828	0.59	+0.5
4,354,489	-		PP_3828	0.59	+0.2
4,354,616	+		PP_3828	0.75	+0.1
4,354,617	-		PP_3828	0.76	-0.1
4,354,617	-		PP_3828	0.76	+0.5
4,354,633	-		PP_3828	0.78	+0.4
4,354,745	+				-0.6
4,354,784	+				+0.5
4,354,784	+				-0.6
4,354,784	+				-0.1
4,354,784	+				-1.5
4,354,784	-				+4.1
4,354,785	-				+0.5
4,354,790	+				-1.6
4,354,791	-				+1.2
4,354,844	+				-0.6
4,354,993	+		PP_3829	0.28	+0.2
4,355,182	-		PP_3829	0.56	+0.9
4,355,205	+		PP_3829	0.59	-0.0
4,355,205	+		PP_3829	0.59	-0.4
4,355,206	-		PP_3829	0.59	-1.1
4,355,206	-		PP_3829	0.59	+2.5
4,355,301	-		PP_3829	0.73	+0.4
4,355,381	-		PP_3829	0.85	-1.0
4,355,697	-		PP_3830	0.20	-0.3
4,355,697	-		PP_3830	0.20	-0.5
4,355,697	-		PP_3830	0.20	+0.0
4,355,730	-		PP_3830	0.23	-0.0
4,355,730	-		PP_3830	0.23	+0.5