Strain Fitness in Pseudomonas putida KT2440 around PP_3801

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,328,679	+		PP_3798	0.85	-0.7
4,328,680	-		PP_3798	0.86	-0.5
4,328,680	-		PP_3798	0.86	-1.1
4,328,766	+				+0.0
4,328,766	+				-1.1
4,328,766	+				-1.6
4,328,793	+		PP_3799	0.12	-1.7
4,328,793	+		PP_3799	0.12	-0.5
4,329,005	+		PP_3799	0.79	-0.8
4,329,005	+		PP_3799	0.79	-0.7
4,329,005	+		PP_3799	0.79	-1.1
4,329,006	-		PP_3799	0.79	+0.9
4,329,006	-		PP_3799	0.79	-0.1
4,329,006	-		PP_3799	0.79	+0.1
4,329,050	+				+1.2
4,329,051	-				-0.2
4,329,121	+				-1.6
4,329,121	+				-0.2
4,329,121	+				-1.9
4,329,121	+				+0.1
4,329,153	-		PP_3800	0.15	-0.6
4,329,153	-		PP_3800	0.15	-1.7
4,329,153	-		PP_3800	0.15	+1.0
4,329,262	+		PP_3800	0.34	+2.5
4,329,263	-		PP_3800	0.34	+0.2
4,329,263	-		PP_3800	0.34	-0.5
4,329,263	-		PP_3800	0.34	-0.6
4,329,300	-		PP_3800	0.40	-1.9
4,329,332	+		PP_3800	0.46	-1.2
4,329,333	-		PP_3800	0.46	-1.1
4,329,604	+				+2.5
4,329,719	-				-0.7
4,329,758	+		PP_3801	0.11	-0.9
4,329,759	-		PP_3801	0.11	-1.7
4,329,759	-		PP_3801	0.11	-0.4
4,329,761	+		PP_3801	0.12	-1.3
4,329,762	-		PP_3801	0.12	-1.9
4,329,762	-		PP_3801	0.12	-1.4
4,329,762	-		PP_3801	0.12	-0.1
4,329,767	+		PP_3801	0.12	-1.5
4,329,767	+		PP_3801	0.12	-0.5
4,329,768	-		PP_3801	0.12	-0.2
4,329,768	-		PP_3801	0.12	+0.1
4,329,768	-		PP_3801	0.12	-3.2
4,329,778	+		PP_3801	0.14	-0.5
4,329,778	+		PP_3801	0.14	-1.5
4,329,779	-		PP_3801	0.14	-0.6
4,329,779	-		PP_3801	0.14	-0.2
4,329,840	-		PP_3801	0.20	-0.3
4,330,133	+		PP_3801	0.53	-1.3
4,330,133	+		PP_3801	0.53	-2.0
4,330,251	-		PP_3801	0.66	-0.3
4,330,256	+		PP_3801	0.66	-1.1
4,330,256	+		PP_3801	0.66	+0.8
4,330,257	-		PP_3801	0.67	-1.5
4,330,409	+		PP_3801	0.83	-0.4
4,330,410	-		PP_3801	0.83	-0.2
4,330,462	+		PP_3801	0.89	+0.2
4,330,462	+		PP_3801	0.89	-0.0
4,330,462	+		PP_3801	0.89	-1.7
4,330,463	-		PP_3801	0.89	+0.3
4,330,463	-		PP_3801	0.89	-0.5
4,330,463	-		PP_3801	0.89	-1.1
4,330,478	+				-1.0
4,330,479	-				+0.1
4,330,493	+				+0.4
4,330,493	+				+0.9
4,330,493	+				-1.5
4,330,494	-				-0.5
4,330,494	-				-1.3
4,330,494	-				-1.0
4,330,801	+		PP_3802	0.33	+0.1
4,330,801	+		PP_3802	0.33	+1.5
4,330,802	-		PP_3802	0.33	-0.3
4,331,105	+		PP_3802	0.74	+0.9
4,331,105	+		PP_3802	0.74	-0.1
4,331,106	-		PP_3802	0.74	-0.8
4,331,106	-		PP_3802	0.74	-0.7
4,331,133	+		PP_3802	0.77	-3.1
4,331,352	+				-2.0
4,331,352	+				-1.2
4,331,353	-				-0.2
4,331,353	-				-0.4
4,331,373	+				-0.7
4,331,373	+				+1.0
4,331,374	-				+2.5
4,331,374	-				-0.7
4,331,535	+		PP_3803	0.26	-1.1
4,331,535	+		PP_3803	0.26	-2.5
4,331,535	+		PP_3803	0.26	-0.7
4,331,535	+		PP_3803	0.26	-0.3
4,331,535	+				+1.5
4,331,538	-		PP_3803	0.26	+0.2
4,331,538	-		PP_3803	0.26	-0.7