Strain Fitness in Pseudomonas putida KT2440 around PP_3779

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,306,161	+		PP_3778	0.11	+0.2
4,306,258	+		PP_3778	0.23	-0.6
4,306,481	+		PP_3778	0.51	+0.1
4,306,482	-		PP_3778	0.52	-0.9
4,306,482	-		PP_3778	0.52	+0.5
4,306,482	-		PP_3778	0.52	-0.7
4,306,482	-		PP_3778	0.52	-0.0
4,306,484	+		PP_3778	0.52	+0.0
4,306,484	+		PP_3778	0.52	+0.3
4,306,484	+		PP_3778	0.52	-0.5
4,306,485	-		PP_3778	0.52	+0.7
4,306,485	-		PP_3778	0.52	+0.0
4,306,519	-		PP_3778	0.56	+0.8
4,306,519	-		PP_3778	0.56	+0.7
4,306,519	-		PP_3778	0.56	-0.2
4,306,552	+		PP_3778	0.60	+0.2
4,306,553	-		PP_3778	0.61	-0.9
4,306,853	+				-0.5
4,306,853	+				-0.7
4,306,868	+				+0.6
4,306,868	+				-0.4
4,306,868	+				-0.0
4,306,868	+				-0.3
4,306,880	-				+1.8
4,306,900	+				+0.8
4,306,956	+				+0.9
4,306,957	-				+1.0
4,306,957	-				+0.4
4,307,069	+				+0.2
4,307,071	+				+0.4
4,307,072	-				+2.5
4,307,102	+				-1.1
4,307,109	+				+0.2
4,307,109	+				-1.0
4,307,109	+				-0.6
4,307,109	+				-0.7
4,307,110	-				+1.5
4,307,110	-				+0.0
4,307,110	-				+1.5
4,307,110	-				+0.7
4,307,113	+				+1.5
4,307,114	-				+2.5
4,307,135	-				-0.1
4,307,161	+				-0.6
4,307,252	+		PP_3779	0.20	+0.7
4,307,252	+		PP_3779	0.20	+1.0
4,307,253	-		PP_3779	0.20	-0.9
4,307,253	-		PP_3779	0.20	-1.0
4,307,253	-		PP_3779	0.20	+0.0
4,307,256	+		PP_3779	0.20	+0.5
4,307,285	-		PP_3779	0.23	+2.5
4,307,315	-		PP_3779	0.26	+0.8
4,307,317	-		PP_3779	0.27	+0.2
4,307,328	+		PP_3779	0.28	-0.5
4,307,362	+		PP_3779	0.32	+1.9
4,307,363	-		PP_3779	0.32	+0.2
4,307,374	+		PP_3779	0.33	+0.7
4,307,438	-		PP_3779	0.40	-1.1
4,307,460	+		PP_3779	0.42	+0.2
4,307,574	-		PP_3779	0.54	-0.1
4,307,588	-		PP_3779	0.56	-0.2
4,307,637	+		PP_3779	0.61	+1.3
4,307,637	+		PP_3779	0.61	+1.1
4,307,780	+		PP_3779	0.76	+1.3
4,307,780	+		PP_3779	0.76	+0.5
4,307,780	+		PP_3779	0.76	+0.7
4,307,781	-		PP_3779	0.76	+0.5
4,307,900	-		PP_3779	0.89	+1.0
4,307,972	-				+1.9
4,307,972	-				-0.6
4,307,977	+				+0.7
4,307,977	+				-0.4
4,307,977	+				-0.6
4,307,977	+				+1.5
4,307,978	-				-1.0
4,307,978	-				-0.2
4,307,978	-				-0.9
4,308,028	+				+1.5
4,308,109	+		PP_3780	0.11	+1.5
4,308,237	+				-0.8
4,308,237	+				-0.3
4,308,238	-				-2.4
4,308,238	-				-0.1
4,308,238	-				-2.2
4,308,239	+				-0.1
4,308,240	-				+0.5
4,308,336	+				-1.3
4,308,337	-				-0.3
4,308,337	-				+0.2
4,308,668	+				+0.1
4,308,767	+		PP_3781	0.12	+1.1
4,308,823	+		PP_3781	0.16	+1.3
4,308,910	+		PP_3781	0.22	+0.9
4,308,910	+		PP_3781	0.22	-0.9
4,308,911	-		PP_3781	0.22	-1.5
4,308,911	-		PP_3781	0.22	+0.4
4,308,939	+		PP_3781	0.24	+0.0
4,308,943	+		PP_3781	0.25	-0.6
4,308,943	+		PP_3781	0.25	+0.6
4,308,943	+		PP_3781	0.25	+1.1
4,308,943	-		PP_3781	0.25	+0.2
4,308,944	-		PP_3781	0.25	+0.9
4,308,944	-		PP_3781	0.25	+0.2
4,308,944	-		PP_3781	0.25	+0.4
4,308,959	+		PP_3781	0.26	-0.0
4,308,960	-		PP_3781	0.26	-0.2