Strain Fitness in Pseudomonas putida KT2440 around PP_3733

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,258,318	+				+0.4
4,258,375	+				-0.1
4,258,398	-				-2.1
4,258,404	+				-1.4
4,258,405	-				-0.1
4,258,405	-				-2.1
4,258,547	+		PP_3732	0.12	-0.2
4,258,547	+		PP_3732	0.12	-0.2
4,258,547	+		PP_3732	0.12	+0.3
4,258,548	-		PP_3732	0.13	+0.0
4,258,548	-		PP_3732	0.13	+0.2
4,258,548	-		PP_3732	0.13	-0.4
4,258,548	-		PP_3732	0.13	+0.1
4,258,548	-		PP_3732	0.13	-0.5
4,259,065	+		PP_3732	0.82	+0.8
4,259,219	+				-0.1
4,259,219	+				+0.5
4,259,219	+				+0.1
4,259,255	+				-2.5
4,259,255	+				-0.3
4,259,255	+				-0.8
4,259,255	+				+0.1
4,259,256	-				+0.1
4,259,258	+				+0.5
4,259,277	-				+0.4
4,259,528	+		PP_3733	0.26	+1.5
4,259,529	-		PP_3733	0.26	-0.9
4,259,717	+		PP_3733	0.44	+0.4
4,259,735	+		PP_3733	0.45	-0.6
4,259,735	+		PP_3733	0.45	+0.9
4,259,736	-		PP_3733	0.46	-0.2
4,259,736	-		PP_3733	0.46	-1.9
4,259,736	-		PP_3733	0.46	-0.6
4,259,738	+		PP_3733	0.46	+2.5
4,259,738	+		PP_3733	0.46	-2.3
4,259,739	-		PP_3733	0.46	+0.1
4,259,739	-		PP_3733	0.46	+0.8
4,259,771	+		PP_3733	0.49	-0.7
4,259,825	+		PP_3733	0.54	-0.7
4,259,857	+		PP_3733	0.57	+1.2
4,259,857	+		PP_3733	0.57	-0.6
4,259,857	+		PP_3733	0.57	+0.8
4,259,857	+		PP_3733	0.57	-0.7
4,260,128	+		PP_3733	0.83	-0.9
4,260,128	+		PP_3733	0.83	-0.7
4,260,128	+		PP_3733	0.83	+0.0
4,260,128	+		PP_3733	0.83	+0.8
4,260,129	-		PP_3733	0.83	+0.1
4,260,129	-		PP_3733	0.83	-0.6
4,260,240	-				-1.5
4,260,240	-				+0.3
4,260,303	+				+0.7
4,260,304	-				+0.9
4,260,304	-				+1.5
4,260,438	+				-0.2
4,260,438	+				-1.3
4,260,438	+				-0.8
4,260,439	-				-1.0
4,260,439	-				-0.6
4,260,527	+				+0.3
4,260,685	+		PP_3734	0.16	+1.5
4,260,686	-		PP_3734	0.16	-2.2
4,260,686	-		PP_3734	0.16	+0.2
4,260,905	+		PP_3734	0.25	-2.1
4,260,905	+		PP_3734	0.25	-0.6
4,260,906	-		PP_3734	0.25	+0.1
4,260,906	-		PP_3734	0.25	+0.3
4,260,906	-		PP_3734	0.25	-1.5
4,260,906	-		PP_3734	0.25	+0.4
4,260,906	-		PP_3734	0.25	-0.1
4,260,906	-		PP_3734	0.25	-0.1
4,260,906	-		PP_3734	0.25	+0.9
4,260,906	-		PP_3734	0.25	-1.3
4,260,936	+		PP_3734	0.26	+0.9
4,260,936	+		PP_3734	0.26	+0.9
4,260,936	+		PP_3734	0.26	-0.3
4,260,937	-		PP_3734	0.26	-0.5
4,261,099	+		PP_3734	0.32	-0.7
4,261,100	-		PP_3734	0.32	-0.2
4,261,220	+		PP_3734	0.37	-1.6
4,261,220	+		PP_3734	0.37	+0.1
4,261,257	+		PP_3734	0.39	-0.2
4,261,257	+		PP_3734	0.39	-0.1
4,261,257	+		PP_3734	0.39	+0.2
4,261,257	+		PP_3734	0.39	+0.3
4,261,258	-		PP_3734	0.39	+0.4
4,261,258	-		PP_3734	0.39	+3.3
4,261,258	-		PP_3734	0.39	-0.9
4,261,258	-		PP_3734	0.39	-0.9