Strain Fitness in Pseudomonas putida KT2440 around PP_3539

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
4,010,773	-				+0.6
4,010,833	-				-0.6
4,010,955	+		PP_3538	0.18	-0.6
4,010,955	+		PP_3538	0.18	-0.3
4,010,955	+		PP_3538	0.18	+2.5
4,010,955	+		PP_3538	0.18	+0.0
4,010,955	+		PP_3538	0.18	+0.9
4,010,955	+		PP_3538	0.18	-0.8
4,010,955	+		PP_3538	0.18	+0.3
4,010,955	+		PP_3538	0.18	-0.1
4,010,955	+		PP_3538	0.18	+0.3
4,010,955	+		PP_3538	0.18	-0.9
4,010,955	+		PP_3538	0.18	+0.2
4,010,956	-		PP_3538	0.19	+0.1
4,010,956	-		PP_3538	0.19	-2.1
4,010,956	-		PP_3538	0.19	+0.1
4,010,956	-		PP_3538	0.19	-0.2
4,010,956	-		PP_3538	0.19	+0.3
4,010,959	+		PP_3538	0.19	-0.3
4,010,960	-		PP_3538	0.19	+0.8
4,010,960	-		PP_3538	0.19	-0.6
4,011,082	-		PP_3538	0.33	+1.0
4,011,082	-		PP_3538	0.33	+0.4
4,011,098	+		PP_3538	0.35	-0.0
4,011,099	-		PP_3538	0.35	-1.0
4,011,109	+		PP_3538	0.36	-0.6
4,011,109	+		PP_3538	0.36	+0.7
4,011,110	-		PP_3538	0.36	+0.0
4,011,110	-		PP_3538	0.36	-0.5
4,011,229	-		PP_3538	0.50	-0.5
4,011,366	+		PP_3538	0.65	-0.4
4,011,367	-		PP_3538	0.65	+0.0
4,011,367	-		PP_3538	0.65	-0.3
4,011,536	+				+2.5
4,011,536	+		PP_3538	0.85	+0.9
4,011,536	+		PP_3538	0.85	+0.2
4,011,536	+		PP_3538	0.85	-0.7
4,011,537	-		PP_3538	0.85	+0.4
4,011,537	-		PP_3538	0.85	+0.5
4,011,540	+		PP_3538	0.85	+0.5
4,011,670	-				+0.4
4,011,708	-				+2.5
4,011,762	+				-0.3
4,011,762	+				+1.5
4,011,762	+				-0.7
4,011,870	+		PP_3539	0.31	-2.2
4,011,870	+		PP_3539	0.31	-0.7
4,011,870	+		PP_3539	0.31	+0.7
4,012,294	+		PP_3540	0.14	+2.5
4,012,458	-		PP_3540	0.33	+4.0
4,013,062	+				+0.3
4,013,062	+				+0.1
4,013,062	+				-0.8
4,013,062	+				-0.0
4,013,063	-				+1.7
4,013,100	+				-1.7
4,013,100	+				-0.3
4,013,101	-				-1.2