Strain Fitness in Pseudomonas putida KT2440 around PP_3508

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
3,979,655	+		PP_3507	0.74	-1.2
3,979,656	-		PP_3507	0.74	-0.3
3,979,838	+		PP_3507	0.79	-1.2
3,980,011	+		PP_3507	0.84	-1.3
3,980,011	+		PP_3507	0.84	-1.4
3,980,012	-		PP_3507	0.84	+0.1
3,980,057	-		PP_3507	0.85	-0.7
3,980,095	+		PP_3507	0.86	-0.3
3,980,096	-		PP_3507	0.86	+1.4
3,980,096	-		PP_3507	0.86	-0.3
3,980,137	+		PP_3507	0.87	-1.5
3,980,137	+		PP_3507	0.87	-0.1
3,980,137	+		PP_3507	0.87	+0.4
3,980,137	+		PP_3507	0.87	+0.1
3,980,419	+				-0.8
3,980,420	-				-2.3
3,980,420	-				-0.4
3,980,778	+		PP_3508	0.13	-1.4
3,980,778	+		PP_3508	0.13	-0.0
3,980,779	-		PP_3508	0.14	+0.1
3,981,354	+		PP_3508	0.67	-2.4
3,981,354	+		PP_3508	0.67	-1.1
3,981,355	-		PP_3508	0.68	-2.3
3,981,923	+				-0.5
3,981,923	+				+0.6
3,981,924	-				-0.2
3,981,924	-				-0.8
3,981,950	+				-1.5
3,981,950	+				-1.6
3,981,950	+				-0.9
3,981,950	+				-0.1
3,981,967	-				-0.8
3,982,073	+				+0.6
3,982,073	+				+0.9
3,982,127	+				-0.7
3,982,127	+				+0.0
3,982,128	-				-0.8
3,982,128	-				-1.5
3,982,142	+				-0.8
3,982,234	+		PP_3509	0.33	+1.1
3,982,234	+		PP_3509	0.33	+0.5
3,982,360	-		PP_3509	0.67	-0.6
3,982,360	-		PP_3509	0.67	+0.0
3,982,360	-		PP_3509	0.67	-1.6
3,982,634	+		PP_3510	0.42	+0.4
3,982,635	-		PP_3510	0.42	-0.1
3,982,635	-		PP_3510	0.42	-0.1
3,982,637	+				+1.4
3,982,637	+		PP_3510	0.43	-1.1
3,982,700	+		PP_3510	0.61	+0.9
3,982,701	-		PP_3510	0.61	+0.4
3,982,701	-				+3.0
3,982,701	-		PP_3510	0.61	+0.1
3,982,701	-		PP_3510	0.61	-0.2
3,982,701	-		PP_3510	0.61	-0.7
3,982,701	-		PP_3510	0.61	-0.4