Strain Fitness in Pseudomonas putida KT2440 around PP_3487

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
3,950,444	-		PP_3486	0.60	+0.1
3,950,446	+		PP_3486	0.60	-0.5
3,950,447	-		PP_3486	0.60	-0.4
3,950,753	+		PP_3486	0.81	+0.2
3,950,910	+				-0.2
3,951,051	+				-0.7
3,951,052	-				+0.6
3,951,228	+				+0.0
3,951,387	-		PP_3487	0.23	-1.7
3,951,387	-		PP_3487	0.23	-1.4
3,951,387	-		PP_3487	0.23	-0.3
3,951,661	+		PP_3487	0.65	-0.6
3,951,941	+				-0.0
3,951,942	-				+0.4
3,952,002	+		PP_3488	0.17	+0.9
3,952,003	-		PP_3488	0.17	-0.1
3,952,005	+		PP_3488	0.17	+0.4
3,952,005	+		PP_3488	0.17	-0.2
3,952,043	+		PP_3488	0.23	+0.2
3,952,043	+		PP_3488	0.23	+0.9
3,952,043	+		PP_3488	0.23	+1.1
3,952,168	-		PP_3488	0.42	-0.2
3,952,296	-		PP_3488	0.61	+1.4
3,952,296	-		PP_3488	0.61	-0.6
3,952,668	-				-0.8
3,952,707	-				+0.4
3,952,707	-				-1.1
3,952,770	-		PP_5606	0.11	+0.1
3,952,824	+		PP_5606	0.14	+1.4
3,952,824	+		PP_5606	0.14	-0.4
3,952,824	+		PP_5606	0.14	-0.3