Strain Fitness in Pseudomonas putida KT2440 around PP_3431

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
3,887,065	+		PP_3430	0.63	+1.6
3,887,329	-		PP_3430	0.75	-0.1
3,887,375	+		PP_3430	0.77	+0.0
3,887,393	-		PP_3430	0.78	-0.4
3,887,421	+		PP_3430	0.79	+0.1
3,887,499	+		PP_3430	0.83	-1.2
3,887,500	-		PP_3430	0.83	-1.3
3,887,500	-		PP_3430	0.83	+0.2
3,887,571	+		PP_3430	0.86	-1.1
3,887,571	+		PP_3430	0.86	-0.1
3,887,571	+		PP_3430	0.86	-0.3
3,887,572	-		PP_3430	0.87	+0.3
3,887,572	-		PP_3430	0.87	+0.1
3,887,689	-				+1.4
3,887,881	+				+0.0
3,887,881	+				+1.0
3,887,927	+				-0.9
3,887,927	+				+1.2
3,887,927	+				+0.0
3,887,927	+				+0.4
3,887,927	+				+0.1
3,887,927	+				+0.9
3,887,927	+				-0.5
3,887,928	-				+0.0
3,887,928	-				-0.0
3,887,928	-				-0.2
3,887,932	+				-1.6
3,887,932	+				-1.0
3,887,932	+				+1.9
3,887,933	-				+0.5
3,887,933	-				-0.6
3,887,937	-				+0.1
3,887,937	-				-0.5
3,888,028	+				-0.3
3,888,028	+				+0.3
3,888,028	+				+2.0
3,888,038	+				+2.1
3,888,038	+				+2.5
3,888,039	-				-0.3
3,888,117	-		PP_3431	0.18	-0.9
3,888,128	+		PP_3431	0.19	-1.0
3,888,128	+		PP_3431	0.19	-0.4
3,888,128	+		PP_3431	0.19	+0.5
3,888,241	+		PP_3431	0.36	-0.7
3,888,242	-		PP_3431	0.36	-0.7
3,888,242	-		PP_3431	0.36	+0.2
3,888,276	-		PP_3431	0.41	-0.2
3,888,276	-		PP_3431	0.41	-0.9
3,888,276	-		PP_3431	0.41	-0.9
3,888,287	+		PP_3431	0.42	-0.8
3,888,288	-		PP_3431	0.43	-0.7
3,888,288	-		PP_3431	0.43	+0.0
3,888,290	+		PP_3431	0.43	-0.5
3,888,291	-		PP_3431	0.43	-0.9
3,888,315	-		PP_3431	0.46	+0.7
3,888,315	-		PP_3431	0.46	-1.2
3,888,402	+		PP_3431	0.59	+2.5
3,888,430	+		PP_3431	0.63	-1.2
3,888,480	+		PP_3431	0.70	-1.0
3,888,480	+		PP_3431	0.70	+0.3
3,888,510	+		PP_3431	0.75	-0.8
3,888,510	+		PP_3431	0.75	-0.3
3,888,511	-		PP_3431	0.75	+1.3
3,888,511	-		PP_3431	0.75	+0.1
3,888,511	-		PP_3431	0.75	-1.0
3,888,511	-		PP_3431	0.75	-1.7
3,888,558	-				-0.3
3,888,588	-		PP_3431	0.86	-0.6
3,888,588	-		PP_3431	0.86	+0.7
3,888,680	+				+1.5
3,888,680	+				-0.7
3,888,684	+				-0.7
3,888,730	-				+0.0
3,888,750	+				-1.4
3,888,751	-				-0.2
3,888,751	-				+1.1
3,888,754	+				-0.5
3,888,754	+				-0.0
3,888,755	-				+0.5
3,888,755	-				+0.9
3,888,803	+				-0.2
3,888,803	+				+0.3
3,888,803	+				+1.1
3,888,803	+				+1.0
3,888,803	+				-0.5
3,888,804	-				+0.3
3,888,804	-				+0.5
3,888,804	-				-0.3
3,888,806	+				+1.2
3,888,806	+				+1.0
3,888,811	+				+0.5
3,888,811	+				-0.2
3,888,811	+				+0.5
3,888,811	+				-1.1
3,888,812	-				-0.5
3,888,812	-				-1.4
3,888,812	-				-0.4
3,888,812	-				-0.1
3,889,045	+		PP_3432	0.29	+2.2
3,889,127	+		PP_3432	0.39	-0.6
3,889,127	+		PP_3432	0.39	-0.3
3,889,128	-		PP_3432	0.39	+0.1
3,889,160	+		PP_3432	0.43	+0.7
3,889,161	-		PP_3432	0.43	+0.0
3,889,202	+		PP_3432	0.48	+0.5
3,889,264	+		PP_3432	0.56	-0.9
3,889,264	+		PP_3432	0.56	+0.2
3,889,264	+		PP_3432	0.56	-0.7
3,889,264	+		PP_3432	0.56	+1.5
3,889,265	-		PP_3432	0.56	-0.5
3,889,283	+		PP_3432	0.58	-0.5
3,889,283	+		PP_3432	0.58	-0.7
3,889,284	-		PP_3432	0.58	+0.1
3,889,284	-		PP_3432	0.58	+0.2
3,889,358	+		PP_3432	0.68	-0.3
3,889,358	+		PP_3432	0.68	-0.3
3,889,358	+		PP_3432	0.68	+0.0
3,889,358	+		PP_3432	0.68	-1.5
3,889,359	-		PP_3432	0.68	+0.7
3,889,359	-		PP_3432	0.68	+0.2
3,889,362	+		PP_3432	0.68	+0.4
3,889,362	+		PP_3432	0.68	-1.1
3,889,362	+		PP_3432	0.68	-0.4
3,889,362	+		PP_3432	0.68	+0.5
3,889,362	+		PP_3432	0.68	-2.6
3,889,417	+		PP_3432	0.75	-0.7
3,889,418	-		PP_3432	0.75	-0.2
3,889,440	-		PP_3432	0.78	-2.1
3,889,445	+		PP_3432	0.78	-0.8
3,889,491	+				+3.0
3,889,628	+				+1.9
3,889,628	+				+0.0
3,889,628	+				-0.2
3,889,670	-				-0.1