Strain Fitness in Pseudomonas putida KT2440 around PP_3365

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
3,807,257	+		PP_3364	0.74	-0.8
3,807,257	+		PP_3364	0.74	+2.5
3,807,257	+		PP_3364	0.74	-1.2
3,807,257	+		PP_3364	0.74	-1.0
3,807,257	+		PP_3364	0.74	+1.3
3,807,258	-		PP_3364	0.74	-3.2
3,807,258	-		PP_3364	0.74	+1.1
3,807,258	-		PP_3364	0.74	-0.5
3,807,360	+		PP_3364	0.78	+2.0
3,807,446	+		PP_3364	0.81	-0.1
3,807,446	+		PP_3364	0.81	-0.4
3,807,447	-		PP_3364	0.81	-0.0
3,807,591	+		PP_3364	0.87	-0.7
3,807,592	-		PP_3364	0.87	+0.1
3,807,592	-		PP_3364	0.87	+1.8
3,807,876	-				-0.0
3,807,951	+				-0.0
3,807,952	-				-1.2
3,807,992	+				+0.3
3,807,992	+				+1.3
3,807,992	+				-0.1
3,807,993	-				-0.2
3,807,994	+				-0.1
3,808,057	-				-0.1
3,808,057	-				+0.3
3,808,230	+		PP_3365	0.11	-1.1
3,809,708	+				-0.5
3,809,709	-				+0.3
3,809,738	+				+0.1
3,809,741	+				-0.5
3,809,741	+				-1.5
3,809,811	+				+1.2
3,809,812	-				-0.4
3,809,872	+				-0.8
3,809,872	+				-1.2
3,809,873	-				+0.6
3,810,161	+		PP_3366	0.31	-0.8
3,810,161	+		PP_3366	0.31	+0.4
3,810,167	+		PP_3366	0.32	-0.2
3,810,167	+		PP_3366	0.32	+0.3
3,810,168	-		PP_3366	0.32	-1.1
3,810,168	-		PP_3366	0.32	-0.2
3,810,168	-		PP_3366	0.32	-1.1
3,810,171	+		PP_3366	0.33	+1.3
3,810,196	+		PP_3366	0.35	+0.3
3,810,197	-		PP_3366	0.35	+1.5
3,810,197	-		PP_3366	0.35	+0.5
3,810,197	-		PP_3366	0.35	-0.8
3,810,197	-		PP_3366	0.35	+2.0
3,810,421	+		PP_3366	0.61	-0.3
3,810,421	+		PP_3366	0.61	+0.8
3,810,422	-		PP_3366	0.61	+0.3
3,810,526	+		PP_3366	0.73	-1.2
3,810,526	+		PP_3366	0.73	-0.5
3,810,540	-		PP_3366	0.74	-0.5