Strain Fitness in Pseudomonas putida KT2440 around PP_2931

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
3,332,104	-				-0.5
3,332,135	+				-2.0
3,332,136	-				+0.1
3,332,139	-				+2.0
3,332,162	+				-0.5
3,332,162	+				+0.6
3,332,163	-				-0.1
3,332,293	+		PP_2930	0.12	-0.3
3,332,293	+		PP_2930	0.12	-0.4
3,332,293	+		PP_2930	0.12	-0.2
3,332,293	+		PP_2930	0.12	-0.0
3,332,293	+		PP_2930	0.12	+3.5
3,332,294	-		PP_2930	0.12	+2.5
3,332,294	-		PP_2930	0.12	+1.3
3,332,294	-		PP_2930	0.12	-0.9
3,332,294	-		PP_2930	0.12	+0.4
3,332,896	+		PP_2930	0.77	-1.2
3,332,983	-		PP_2930	0.87	+1.3
3,333,041	+				+1.3
3,333,041	+				+1.3
3,333,041	+				+0.3
3,333,042	-				+0.2
3,333,042	-				+0.9
3,333,119	+				+0.8
3,333,119	+				-0.1
3,333,119	+				+1.8
3,333,138	+				-0.4
3,333,138	+				-1.3
3,333,138	+				+1.2
3,333,138	+				-0.1
3,333,138	+				+2.5
3,333,139	-				+0.4
3,333,139	-				-0.2
3,333,139	-				+0.8
3,333,147	+				-0.2
3,333,148	-				-0.4
3,333,341	+		PP_2931	0.34	-1.2
3,333,342	-		PP_2931	0.34	-0.1
3,333,491	-		PP_2931	0.55	-1.0
3,333,491	-		PP_2931	0.55	-0.8
3,333,770	-				+1.6
3,333,853	-				-1.8
3,333,949	+				-1.7
3,333,984	-				+1.0
3,333,984	-				-1.1
3,334,285	+		PP_2932	0.25	-0.9
3,334,285	+		PP_2932	0.25	-0.3
3,334,285	+		PP_2932	0.25	-0.6
3,334,285	+		PP_2932	0.25	+1.5
3,334,286	-		PP_2932	0.25	+0.5
3,334,383	-		PP_2932	0.31	+0.4
3,334,383	-		PP_2932	0.31	-0.4
3,334,486	+		PP_2932	0.37	-0.1
3,334,551	+		PP_2932	0.41	+0.5
3,334,689	+		PP_2932	0.49	-0.7
3,334,689	+		PP_2932	0.49	-1.7
3,334,690	-		PP_2932	0.49	+0.0
3,334,690	-		PP_2932	0.49	+0.4
3,334,691	+		PP_2932	0.49	+1.0
3,334,692	-		PP_2932	0.49	+0.2
3,334,692	-		PP_2932	0.49	-0.1
3,334,693	+		PP_2932	0.49	-1.0
3,334,693	+				+2.5
3,334,693	+		PP_2932	0.49	-0.3
3,334,693	+		PP_2932	0.49	-2.0
3,334,693	+		PP_2932	0.49	-1.9
3,334,694	-		PP_2932	0.49	-0.8
3,334,694	-		PP_2932	0.49	+0.2
3,334,694	-		PP_2932	0.49	-0.5
3,334,771	-		PP_2932	0.54	-0.7