Strain Fitness in Pseudomonas putida KT2440 around PP_2880

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
3,279,066	+		PP_2877	0.84	+0.4
3,279,149	+				-1.2
3,279,149	+				-0.1
3,279,150	-				-0.5
3,279,150	-				+0.3
3,279,184	+				-1.8
3,279,184	+				-1.0
3,279,185	-				-0.3
3,279,237	-				+1.9
3,279,248	+				+0.2
3,279,248	+				-0.4
3,279,248	+				-0.7
3,279,249	-				+0.3
3,279,249	-				+2.1
3,279,308	-		PP_2878	0.31	-0.5
3,279,388	-		PP_2878	0.75	+0.3
3,279,461	+				-0.4
3,279,462	-				-0.1
3,279,462	-				+0.5
3,279,581	+		PP_2879	0.16	-1.9
3,279,581	+		PP_2879	0.16	-0.7
3,279,583	+		PP_2879	0.16	+0.6
3,279,584	-		PP_2879	0.16	+0.3
3,279,584	-		PP_2879	0.16	-0.3
3,279,619	+		PP_2879	0.22	+0.6
3,279,619	+		PP_2879	0.22	-0.9
3,279,620	-		PP_2879	0.23	+0.4
3,279,620	-		PP_2879	0.23	+0.4
3,279,711	-		PP_2879	0.38	-0.8
3,279,711	-		PP_2879	0.38	-0.4
3,279,711	-		PP_2879	0.38	+0.9
3,279,739	-		PP_2879	0.43	+0.6
3,279,836	+		PP_2879	0.59	-0.4
3,279,837	-		PP_2879	0.59	-0.2
3,279,837	-		PP_2879	0.59	-0.7
3,279,899	-		PP_2879	0.70	-0.9
3,279,899	-				+0.5
3,280,000	+		PP_2879	0.87	-0.6
3,280,000	+		PP_2879	0.87	+0.8
3,280,067	+				+0.6
3,280,118	-				+0.1
3,280,243	+				+1.5
3,280,243	+		PP_2880	0.31	-0.5
3,280,455	+		PP_2880	0.69	-0.5
3,280,487	+		PP_2880	0.75	-0.1
3,280,672	-				+0.5
3,280,676	+				+0.1
3,280,676	+				+0.1
3,280,702	+				-0.5
3,280,806	+		PP_2881	0.12	-2.5
3,280,806	+		PP_2881	0.12	+0.6
3,280,806	+		PP_2881	0.12	-1.0
3,280,807	-		PP_2881	0.12	+0.0
3,280,808	+		PP_2881	0.12	+0.3
3,280,808	+		PP_2881	0.12	-0.3
3,280,809	-		PP_2881	0.12	-0.5
3,280,809	-		PP_2881	0.12	+0.6
3,280,809	-		PP_2881	0.12	+0.1
3,280,809	-		PP_2881	0.12	-0.1
3,280,809	-		PP_2881	0.12	-0.2
3,280,809	-		PP_2881	0.12	+0.4
3,280,944	-		PP_2881	0.31	-0.1
3,280,955	+		PP_2881	0.32	-1.2
3,280,955	+		PP_2881	0.32	-1.2
3,280,957	+		PP_2881	0.33	-0.7
3,280,958	-		PP_2881	0.33	-0.8
3,281,373	+		PP_2881	0.89	-0.4
3,281,374	-		PP_2881	0.89	+1.2
3,281,374	-		PP_2881	0.89	+0.2
3,281,448	+				-0.3
3,281,448	+				+0.1
3,281,528	-				+0.7