Strain Fitness in Pseudomonas putida KT2440 around PP_2856

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
3,257,788	-		PP_2854	0.78	-0.3
3,257,788	-		PP_2854	0.78	-0.3
3,257,834	+		PP_2854	0.84	-0.9
3,257,953	-				-0.1
3,257,987	+				+1.0
3,258,188	+		PP_2855	0.28	+0.3
3,258,189	-		PP_2855	0.28	-0.1
3,258,189	-		PP_2855	0.28	+0.2
3,258,221	+		PP_2855	0.33	+0.4
3,258,221	+		PP_2855	0.33	-0.5
3,258,222	-		PP_2855	0.33	+0.8
3,258,222	-		PP_2855	0.33	-0.9
3,258,222	-		PP_2855	0.33	-0.5
3,258,266	+		PP_2855	0.39	+0.5
3,258,289	+		PP_2855	0.43	-0.3
3,258,473	+		PP_2855	0.70	+0.6
3,258,474	-		PP_2855	0.70	-0.4
3,258,692	-				+1.5
3,258,801	-				-0.8
3,258,862	+				+0.2
3,258,910	+		PP_2856	0.12	+0.9
3,258,911	-		PP_2856	0.12	+0.8
3,258,911	-		PP_2856	0.12	+1.4
3,258,911	-		PP_2856	0.12	-0.6
3,258,911	-		PP_2856	0.12	-0.7
3,258,911	-		PP_2856	0.12	+0.9
3,258,925	-		PP_2856	0.13	+4.1
3,259,021	-		PP_2856	0.19	+0.5
3,259,045	-		PP_2856	0.21	-0.1
3,259,092	+		PP_2856	0.24	-0.3
3,259,114	-		PP_2856	0.26	+1.2
3,259,129	-				-0.7
3,259,231	+		PP_2856	0.34	+1.5
3,259,231	+		PP_2856	0.34	+1.4
3,259,232	-		PP_2856	0.34	+1.7
3,259,238	-		PP_2856	0.34	-0.2
3,259,532	-		PP_2856	0.55	-1.3
3,259,548	+		PP_2856	0.56	+0.7
3,259,549	-		PP_2856	0.56	+1.0
3,259,571	+		PP_2856	0.57	-1.8
3,259,758	+		PP_2856	0.70	+1.0
3,259,758	+		PP_2856	0.70	+1.0
3,259,759	-		PP_2856	0.70	+1.6
3,259,913	+		PP_2856	0.81	-0.3
3,259,913	+		PP_2856	0.81	-1.4
3,259,913	+		PP_2856	0.81	-0.2
3,260,034	+		PP_2856	0.89	+0.2
3,260,077	+				+0.5
3,260,078	-				+0.3
3,260,078	-				+0.6
3,260,101	+				+0.2
3,260,126	+				+0.9
3,260,127	-				+0.5
3,260,209	+				+0.6
3,260,210	-				-0.4
3,260,212	+				-0.0
3,260,231	-				+0.5
3,260,348	+		PP_2857	0.18	-0.7
3,260,710	+		PP_2857	0.81	-0.1
3,260,896	-		PP_2858	0.17	+0.1
3,261,096	+		PP_2858	0.63	-0.1