Strain Fitness in Pseudomonas putida KT2440 around PP_2388

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,726,140	-		PP_2387	0.52	+1.4
2,726,163	-		PP_2387	0.54	-1.9
2,726,163	-		PP_2387	0.54	-0.3
2,726,565	+				-0.8
2,726,567	+				+0.4
2,726,567	+				+0.9
2,726,606	+				+1.0
2,726,606	+				+0.8
2,726,721	-				-0.5
2,726,822	+		PP_2388	0.20	-2.0
2,726,822	+		PP_2388	0.20	-1.2
2,726,822	+		PP_2388	0.20	-2.7
2,726,823	-		PP_2388	0.20	-1.2
2,726,823	-		PP_2388	0.20	-2.4
2,727,069	-		PP_2388	0.60	-1.5
2,727,277	+				-1.5
2,727,277	+				-0.8
2,727,278	-				-2.1
2,727,297	-				-2.7
2,727,297	-				-0.1
2,727,316	-				-2.3
2,727,337	+				-2.5
2,727,339	+				-1.5
2,727,340	-				-1.5
2,727,436	+		PP_2389	0.14	+0.1
2,727,437	-				+1.3
2,727,490	+		PP_2389	0.21	-1.2
2,727,759	+		PP_2389	0.55	-0.7
2,727,760	-		PP_2389	0.55	+1.4
2,727,760	-		PP_2389	0.55	+0.4
2,727,877	+		PP_2389	0.70	-1.2
2,727,877	+		PP_2389	0.70	-1.4
2,727,877	+		PP_2389	0.70	-2.6
2,727,877	+		PP_2389	0.70	-0.9
2,727,878	-		PP_2389	0.70	-0.3
2,727,878	-		PP_2389	0.70	+0.1
2,727,878	-		PP_2389	0.70	+0.5
2,727,878	-		PP_2389	0.70	+0.8
2,727,878	-		PP_2389	0.70	-0.4
2,727,878	-		PP_2389	0.70	+0.4
2,727,954	+		PP_2389	0.80	-0.6
2,728,001	+		PP_2389	0.86	-1.1
2,728,018	-		PP_2389	0.88	-1.0
2,728,232	+				-2.3
2,728,240	-				-0.3
2,728,246	-				+0.6