Strain Fitness in Pseudomonas putida KT2440 around PP_2371

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,706,613	-				-0.1
2,706,668	+				-0.0
2,706,668	+				-1.0
2,706,668	+				-0.2
2,706,669	-				-0.1
2,706,825	+				-0.7
2,706,825	+				-0.3
2,706,826	-				+0.1
2,706,930	+				-0.4
2,706,931	-				-1.0
2,706,950	+				+0.2
2,707,125	-				+0.9
2,707,125	-				-1.0
2,707,304	+		PP_2370	0.46	-0.9
2,707,405	+		PP_2370	0.66	-2.0
2,707,405	+		PP_2370	0.66	+0.1
2,707,406	-		PP_2370	0.66	-2.2
2,707,406	-		PP_2370	0.66	-0.9
2,707,476	-		PP_2370	0.80	+1.5
2,707,557	+				-1.2
2,707,572	+				-1.9
2,707,572	+				-1.3
2,707,573	-				-2.9
2,707,604	-				-2.1
2,707,611	+				-1.0
2,707,611	+				-1.2
2,707,611	+				+0.3
2,707,612	-				-1.1
2,707,640	-				-0.4
2,707,768	-		PP_2371	0.13	-0.8
2,707,768	-		PP_2371	0.13	-2.0
2,707,884	+		PP_2371	0.20	-2.3
2,707,885	-		PP_2371	0.20	-1.7
2,707,885	-		PP_2371	0.20	+0.9
2,707,885	-		PP_2371	0.20	+0.9
2,708,118	+		PP_2371	0.34	+2.5
2,708,118	+		PP_2371	0.34	-2.2
2,708,316	+		PP_2371	0.46	-1.2
2,708,470	-		PP_2371	0.55	-2.6
2,708,470	-		PP_2371	0.55	-1.9
2,708,600	+		PP_2371	0.63	-1.4
2,708,600	+		PP_2371	0.63	+2.5
2,708,600	+		PP_2371	0.63	+0.1
2,708,727	+		PP_2371	0.71	-0.3
2,708,976	+		PP_2371	0.86	-1.5
2,708,977	-		PP_2371	0.86	-2.3
2,709,072	+				+1.5
2,709,072	+				+2.5
2,709,072	+				-0.1
2,709,072	+				-0.0
2,709,072	+				-0.1
2,709,072	+				-2.5
2,709,072	+				-2.1
2,709,072	+				-1.4
2,709,072	+				-0.7
2,709,072	+				-0.5
2,709,072	+				+0.1
2,709,073	-				-1.2
2,709,073	-				+2.5
2,709,073	-				-0.7
2,709,073	-				-1.2
2,709,073	-				+1.5
2,709,075	-				+0.9
2,709,075	-				+1.5
2,709,198	+				-2.6
2,709,199	-				-0.7
2,709,200	+				-1.5
2,709,200	+				-2.1
2,709,200	+				-1.5
2,709,201	-				-0.1
2,709,201	-				-0.1
2,709,201	-				-1.1
2,709,201	-				+1.5
2,709,205	-				+1.5
2,709,205	-				-2.3
2,709,205	-				+0.9
2,709,205	-				-0.9
2,709,208	+				+0.5
2,709,208	+				-1.0
2,709,234	+				-0.1
2,709,280	+				-0.5
2,709,281	-				-0.2
2,709,416	+				+0.4
2,709,416	+				+1.5
2,709,539	+		PP_2372	0.11	-0.3
2,709,539	+		PP_2372	0.11	-0.1
2,709,539	+		PP_2372	0.11	+1.0
2,709,539	+		PP_2372	0.11	+0.3
2,709,539	+		PP_2372	0.11	-0.4
2,709,540	-		PP_2372	0.11	-0.4
2,709,540	-		PP_2372	0.11	-0.7
2,709,540	-		PP_2372	0.11	+1.6
2,709,540	-		PP_2372	0.11	-0.9
2,709,596	+		PP_2372	0.16	-0.2
2,709,596	+		PP_2372	0.16	+1.5
2,709,596	+		PP_2372	0.16	-0.6
2,709,596	+		PP_2372	0.16	+0.2
2,709,597	-		PP_2372	0.16	-0.6
2,709,597	-		PP_2372	0.16	+0.6
2,709,598	+		PP_2372	0.16	-0.2
2,709,598	+		PP_2372	0.16	+0.5
2,709,692	+		PP_2372	0.25	+0.2
2,709,692	+		PP_2372	0.25	-0.3
2,709,693	-		PP_2372	0.25	-0.0
2,709,693	-		PP_2372	0.25	-0.4
2,709,806	+		PP_2372	0.36	+1.5
2,709,807	-		PP_2372	0.36	-0.5
2,709,807	-		PP_2372	0.36	-0.4
2,709,807	-		PP_2372	0.36	+0.1
2,709,823	+		PP_2372	0.37	-0.9
2,709,823	+		PP_2372	0.37	+0.4
2,710,020	-		PP_2372	0.56	-0.2
2,710,046	+		PP_2372	0.58	-1.2
2,710,047	-		PP_2372	0.58	+0.2