Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2369 and PP_2370 are separated by 46 nucleotides PP_2370 and PP_2371 overlap by 17 nucleotides
PP_2369: PP_2369 - Leucine-rich repeat domain protein, at 2,702,844 to 2,707,031
_2369
PP_2370: PP_2370 - conserved protein of unknown function, at 2,707,078 to 2,707,572
_2370
PP_2371: PP_2371 - Sulphite reductase hemoprotein, beta subunit, at 2,707,556 to 2,709,208
_2371
Position (kb)
2707
2708 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2706.167 kb on - strand, within PP_2369 at 2706.311 kb on - strand at 2706.328 kb on - strand, within PP_2369 at 2706.328 kb on - strand, within PP_2369 at 2706.368 kb on + strand, within PP_2369 at 2706.368 kb on + strand, within PP_2369 at 2706.400 kb on + strand at 2706.400 kb on + strand, within PP_2369 at 2706.524 kb on - strand, within PP_2369 at 2706.613 kb on - strand at 2706.668 kb on + strand at 2706.668 kb on + strand at 2706.668 kb on + strand at 2706.669 kb on - strand at 2706.825 kb on + strand at 2706.825 kb on + strand at 2706.826 kb on - strand at 2706.930 kb on + strand at 2706.931 kb on - strand at 2706.950 kb on + strand at 2707.125 kb on - strand at 2707.125 kb on - strand at 2707.304 kb on + strand, within PP_2370 at 2707.405 kb on + strand, within PP_2370 at 2707.405 kb on + strand, within PP_2370 at 2707.406 kb on - strand, within PP_2370 at 2707.406 kb on - strand, within PP_2370 at 2707.476 kb on - strand, within PP_2370 at 2707.557 kb on + strand at 2707.572 kb on + strand at 2707.572 kb on + strand at 2707.573 kb on - strand at 2707.604 kb on - strand at 2707.611 kb on + strand at 2707.611 kb on + strand at 2707.611 kb on + strand at 2707.612 kb on - strand at 2707.640 kb on - strand at 2707.768 kb on - strand, within PP_2371 at 2707.768 kb on - strand, within PP_2371 at 2707.884 kb on + strand, within PP_2371 at 2707.885 kb on - strand, within PP_2371 at 2707.885 kb on - strand, within PP_2371 at 2707.885 kb on - strand, within PP_2371 at 2708.118 kb on + strand, within PP_2371 at 2708.118 kb on + strand, within PP_2371 at 2708.316 kb on + strand, within PP_2371 at 2708.470 kb on - strand, within PP_2371 at 2708.470 kb on - strand, within PP_2371
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 2,706,167 - PP_2369 0.79 -0.9 2,706,311 - -0.4 2,706,328 - PP_2369 0.83 +0.3 2,706,328 - PP_2369 0.83 +0.1 2,706,368 + PP_2369 0.84 -0.5 2,706,368 + PP_2369 0.84 +0.1 2,706,400 + +0.5 2,706,400 + PP_2369 0.85 -0.6 2,706,524 - PP_2369 0.88 +0.5 2,706,613 - -0.1 2,706,668 + -0.0 2,706,668 + -1.0 2,706,668 + -0.2 2,706,669 - -0.1 2,706,825 + -0.7 2,706,825 + -0.3 2,706,826 - +0.1 2,706,930 + -0.4 2,706,931 - -1.0 2,706,950 + +0.2 2,707,125 - +0.9 2,707,125 - -1.0 2,707,304 + PP_2370 0.46 -0.9 2,707,405 + PP_2370 0.66 -2.0 2,707,405 + PP_2370 0.66 +0.1 2,707,406 - PP_2370 0.66 -2.2 2,707,406 - PP_2370 0.66 -0.9 2,707,476 - PP_2370 0.80 +1.5 2,707,557 + -1.2 2,707,572 + -1.9 2,707,572 + -1.3 2,707,573 - -2.9 2,707,604 - -2.1 2,707,611 + -1.0 2,707,611 + -1.2 2,707,611 + +0.3 2,707,612 - -1.1 2,707,640 - -0.4 2,707,768 - PP_2371 0.13 -0.8 2,707,768 - PP_2371 0.13 -2.0 2,707,884 + PP_2371 0.20 -2.3 2,707,885 - PP_2371 0.20 -1.7 2,707,885 - PP_2371 0.20 +0.9 2,707,885 - PP_2371 0.20 +0.9 2,708,118 + PP_2371 0.34 +2.5 2,708,118 + PP_2371 0.34 -2.2 2,708,316 + PP_2371 0.46 -1.2 2,708,470 - PP_2371 0.55 -2.6 2,708,470 - PP_2371 0.55 -1.9
Or see this region's nucleotide sequence