Strain Fitness in Pseudomonas putida KT2440 around PP_2356

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,687,347	+		PP_2354	0.84	+1.1
2,687,408	+		PP_2354	0.87	-0.2
2,687,408	+		PP_2354	0.87	-0.4
2,687,409	-		PP_2354	0.87	-0.2
2,687,457	-		PP_2354	0.89	-1.5
2,687,473	+		PP_2354	0.90	-0.5
2,687,474	-		PP_2354	0.90	+0.1
2,687,474	-		PP_2354	0.90	-1.7
2,687,567	+				+0.2
2,687,661	+				+0.6
2,687,662	-				-0.2
2,687,698	+				-0.1
2,687,698	+				-1.5
2,687,699	-				+0.6
2,687,801	+		PP_2355	0.14	+0.1
2,687,859	+		PP_2355	0.27	+0.3
2,687,860	-		PP_2355	0.27	-0.3
2,687,860	-		PP_2355	0.27	-0.3
2,687,860	-		PP_2355	0.27	-0.6
2,687,860	-		PP_2355	0.27	+0.2
2,688,011	+		PP_2355	0.60	+0.1
2,688,048	+		PP_2355	0.68	-0.0
2,688,069	+		PP_2355	0.72	+0.9
2,688,070	-		PP_2355	0.73	-0.4
2,688,202	+				+0.5
2,688,217	+				+0.6
2,688,217	+				+0.1
2,688,217	+				+0.6
2,688,218	-				-2.9
2,688,314	+				-1.5
2,688,315	-				+0.9
2,688,315	-				+0.5
2,688,315	-				+0.9
2,688,458	+		PP_2356	0.11	+0.5
2,688,459	-		PP_2356	0.11	+0.1
2,688,488	-				+2.5
2,688,501	+		PP_2356	0.13	-0.9
2,688,501	+		PP_2356	0.13	-2.6
2,688,502	-		PP_2356	0.13	+0.5
2,688,609	+		PP_2356	0.18	-1.9
2,688,609	+		PP_2356	0.18	+0.1
2,688,610	-		PP_2356	0.18	-0.5
2,688,751	+		PP_2356	0.24	+0.1
2,688,818	-		PP_2356	0.27	+0.1
2,688,867	+		PP_2356	0.29	-1.5
2,688,975	-		PP_2356	0.34	-0.7
2,689,119	-		PP_2356	0.40	-1.9
2,689,119	-		PP_2356	0.40	-0.7
2,689,146	+		PP_2356	0.41	-1.4
2,689,146	+		PP_2356	0.41	-1.4
2,689,147	-		PP_2356	0.41	-0.1
2,689,250	+		PP_2356	0.46	+0.4
2,689,250	+		PP_2356	0.46	-2.8
2,689,251	-		PP_2356	0.46	-2.0
2,689,251	-		PP_2356	0.46	-0.1
2,689,275	-		PP_2356	0.47	-1.1
2,689,415	+		PP_2356	0.53	-1.1
2,689,415	+		PP_2356	0.53	-0.7
2,689,416	-		PP_2356	0.53	-0.7
2,689,416	-		PP_2356	0.53	-0.7
2,689,416	-		PP_2356	0.53	+0.9
2,689,511	+		PP_2356	0.57	-0.6
2,689,512	-				-0.4
2,689,512	-		PP_2356	0.57	+0.4
2,689,671	+		PP_2356	0.64	-2.7
2,689,672	-		PP_2356	0.64	-0.8
2,689,672	-		PP_2356	0.64	-1.5
2,689,672	-		PP_2356	0.64	-2.1
2,689,692	-		PP_2356	0.65	-2.2
2,689,696	+		PP_2356	0.65	-0.1
2,689,697	-		PP_2356	0.65	-0.0
2,689,697	-		PP_2356	0.65	-0.7
2,689,697	-		PP_2356	0.65	-1.9
2,689,773	+				+0.9
2,689,889	+		PP_2356	0.74	-1.3
2,690,003	-		PP_2356	0.79	-2.3
2,690,049	+		PP_2356	0.81	+0.5
2,690,052	+		PP_2356	0.81	+1.7
2,690,111	+		PP_2356	0.84	-2.0
2,690,491	+				-0.5
2,690,492	-				+0.0
2,690,602	+				-0.9
2,690,602	+				+0.5
2,690,643	+				+0.1
2,690,644	-				+1.5
2,690,644	-				+0.3
2,690,644	-				+0.2
2,690,661	-				-0.6
2,690,690	+				+0.6
2,690,690	+				-0.2
2,690,721	-		PP_2357	0.10	+0.0
2,690,880	-		PP_2357	0.37	-0.2
2,690,986	-		PP_2357	0.55	-0.4
2,691,037	-		PP_2357	0.63	+1.1
2,691,085	-		PP_2357	0.71	-0.2
2,691,174	+		PP_2357	0.86	-0.9
2,691,175	-		PP_2357	0.87	+0.9
2,691,175	-		PP_2357	0.87	-1.1
2,691,175	-		PP_2357	0.87	+0.2
2,691,182	+		PP_2357	0.88	-0.6
2,691,220	-				+0.5
2,691,264	+				+0.5