Strain Fitness in Pseudomonas putida KT2440 around PP_2345

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,675,375	-		PP_2344	0.30	+0.3
2,675,392	+		PP_2344	0.31	+0.1
2,675,393	-		PP_2344	0.31	+0.4
2,675,393	-		PP_2344	0.31	-0.3
2,675,393	-		PP_2344	0.31	-0.4
2,675,525	-		PP_2344	0.44	-0.5
2,675,842	+		PP_2344	0.73	+1.4
2,675,842	+		PP_2344	0.73	+0.0
2,675,842	+		PP_2344	0.73	+0.9
2,675,844	+		PP_2344	0.73	-0.2
2,675,844	+		PP_2344	0.73	-0.9
2,675,844	+		PP_2344	0.73	+0.4
2,675,845	-		PP_2344	0.73	-2.0
2,675,845	-		PP_2344	0.73	-0.4
2,675,845	-		PP_2344	0.73	+0.4
2,675,845	-		PP_2344	0.73	-1.7
2,675,845	-		PP_2344	0.73	+0.6
2,675,845	-		PP_2344	0.73	-0.2
2,676,152	+				-2.4
2,676,152	+				+0.2
2,676,153	-				+0.8
2,676,188	+				+0.7
2,676,188	+				-0.1
2,676,188	+				-0.9
2,676,189	-				+0.3
2,676,189	-				-2.2
2,676,189	-				+0.1
2,676,189	-				+0.5
2,676,189	-				-0.2
2,676,199	+				-0.0
2,676,199	+				-0.7
2,676,199	+				-0.7
2,676,199	+				+1.1
2,676,200	-				+1.0
2,676,200	-				+0.3
2,676,200	-				+0.7
2,676,200	-				-0.7
2,676,200	-				-1.4
2,676,210	-				+0.1
2,676,210	-				+0.5
2,676,270	-				-0.3
2,676,309	+				-1.9
2,676,309	+				+0.2
2,676,310	-				-0.4
2,676,310	-				-1.4
2,676,310	-				-0.4
2,676,323	+				-0.1
2,676,324	-				+0.8
2,676,570	+		PP_2345	0.64	-0.6
2,676,580	-		PP_2345	0.67	-2.4
2,676,580	-		PP_2345	0.67	-0.4
2,676,580	-		PP_2345	0.67	-1.6
2,676,585	+		PP_2345	0.69	-0.9
2,676,585	+				+1.4
2,677,024	-				-1.0
2,677,024	-				-0.4
2,677,068	-		PP_2347	0.11	+0.6
2,677,107	-		PP_2347	0.17	-0.6
2,677,218	+		PP_2347	0.33	+0.8
2,677,287	-		PP_2347	0.44	-0.4
2,677,287	-		PP_2347	0.44	+0.5
2,677,475	+		PP_2347	0.72	-0.0
2,677,532	+		PP_2347	0.80	-1.1
2,677,533	-		PP_2347	0.80	+0.2
2,677,533	-		PP_2347	0.80	-0.9
2,677,637	+				+0.4
2,677,637	+				+1.0
2,677,637	+				-1.4
2,677,637	+				-0.3
2,677,638	-				+0.3
2,677,638	-				-0.4
2,677,638	-				-0.4
2,677,638	-				+0.9
2,677,643	+				+0.4
2,677,643	+				+0.2
2,677,644	-				-0.4
2,677,644	-				-0.2
2,677,644	-				-1.8
2,677,644	-				+2.4