Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2151 and PP_2152 are separated by 48 nucleotides PP_2152 and PP_2153 are separated by 31 nucleotides PP_2153 and PP_2154 are separated by 198 nucleotides
PP_2151: PP_2151 - soluble pyridine nucleotide transhydrogenase, at 2,458,133 to 2,459,527
_2151
PP_2152: PP_2152 - glycerophosphoryl diester phosphodiesterase, at 2,459,576 to 2,460,292
_2152
PP_2153: PP_2153 - conserved protein of unknown function, at 2,460,324 to 2,460,803
_2153
PP_2154: PP_2154 - LolC, at 2,461,002 to 2,462,252
_2154
Position (kb)
2459
2460
2461 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2459.614 kb on + strand at 2459.767 kb on - strand, within PP_2152 at 2459.858 kb on + strand, within PP_2152 at 2459.904 kb on + strand, within PP_2152 at 2459.904 kb on + strand, within PP_2152 at 2460.279 kb on + strand at 2460.280 kb on - strand at 2460.388 kb on + strand, within PP_2153 at 2460.389 kb on - strand, within PP_2153 at 2460.389 kb on - strand, within PP_2153 at 2460.717 kb on + strand, within PP_2153 at 2460.717 kb on + strand, within PP_2153 at 2460.840 kb on + strand at 2460.840 kb on + strand at 2460.840 kb on + strand at 2460.871 kb on + strand at 2460.897 kb on + strand at 2460.897 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO. remove 2,459,614 + +2.4 2,459,767 - PP_2152 0.27 +0.0 2,459,858 + PP_2152 0.39 -0.7 2,459,904 + PP_2152 0.46 -1.8 2,459,904 + PP_2152 0.46 -1.0 2,460,279 + +0.8 2,460,280 - -2.2 2,460,388 + PP_2153 0.13 -0.5 2,460,389 - PP_2153 0.14 +0.4 2,460,389 - PP_2153 0.14 +0.1 2,460,717 + PP_2153 0.82 -0.2 2,460,717 + PP_2153 0.82 -0.4 2,460,840 + -0.4 2,460,840 + +0.7 2,460,840 + -0.5 2,460,871 + +0.1 2,460,897 + -0.9 2,460,897 + +0.1
Or see this region's nucleotide sequence