Strain Fitness in Pseudomonas putida KT2440 around PP_2142

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,443,434	+				+1.4
2,443,435	-				+2.4
2,443,464	-				+0.9
2,443,518	+				-1.4
2,443,529	+				-0.3
2,443,529	+				+0.3
2,443,540	+				-0.3
2,443,540	+				-1.1
2,443,540	+				+0.2
2,443,541	-				+0.6
2,443,578	+		PP_2140	0.16	+0.4
2,443,710	+		PP_2140	0.38	+0.4
2,443,710	+		PP_2140	0.38	+0.0
2,443,711	-		PP_2140	0.38	-0.7
2,443,711	-		PP_2140	0.38	-0.6
2,443,711	-		PP_2140	0.38	-1.0
2,443,752	+		PP_2140	0.45	-0.2
2,443,811	+		PP_2140	0.55	+1.5
2,443,891	-		PP_2140	0.68	+1.8
2,443,891	-		PP_2140	0.68	-0.8
2,444,034	-				-1.2
2,444,096	-				+0.4
2,444,101	-				+1.2
2,444,128	+				+1.4
2,444,128	+				+1.2
2,444,128	+				-0.2
2,444,128	+				+0.8
2,444,128	+				-0.0
2,444,129	-				+1.6
2,444,129	-				-0.0
2,444,129	-				-0.1
2,444,129	-				+3.2
2,444,129	-				+0.6
2,444,135	-				+0.5
2,444,377	-				-0.5
2,444,465	-		PP_2142	0.11	+2.4
2,444,595	+		PP_2142	0.38	-1.2
2,444,595	+		PP_2142	0.38	-0.7
2,444,596	-		PP_2142	0.38	-1.2
2,445,156	-		PP_2143	0.42	+2.4
2,445,169	-		PP_2143	0.44	+2.4
2,445,549	-				-0.3
2,445,682	+				+3.4
2,445,713	+				+0.3
2,445,723	-				+1.4
2,445,819	+		PP_2144	0.13	+0.3
2,445,819	+		PP_2144	0.13	-0.6