Strain Fitness in Pseudomonas putida KT2440 around PP_2020

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,293,016	+		PP_2019	0.59	-0.5
2,293,050	-				+1.7
2,293,050	-		PP_2019	0.60	+0.3
2,293,154	+		PP_2019	0.65	-0.8
2,293,304	+		PP_2019	0.71	-0.3
2,293,517	+		PP_2019	0.80	-0.3
2,293,518	-		PP_2019	0.80	-0.1
2,293,518	-		PP_2019	0.80	+1.7
2,293,584	+		PP_2019	0.82	+0.8
2,293,584	+		PP_2019	0.82	+0.4
2,293,628	+		PP_2019	0.84	-0.6
2,293,628	+		PP_2019	0.84	-0.3
2,293,628	+		PP_2019	0.84	+0.4
2,293,628	+		PP_2019	0.84	+1.2
2,293,628	+		PP_2019	0.84	+1.5
2,293,629	-		PP_2019	0.84	+1.3
2,293,629	-		PP_2019	0.84	+0.7
2,293,629	-		PP_2019	0.84	+0.3
2,293,629	-		PP_2019	0.84	-0.6
2,293,697	+		PP_2019	0.87	+0.5
2,293,697	+		PP_2019	0.87	+1.1
2,293,698	-		PP_2019	0.87	+0.0
2,293,706	+		PP_2019	0.88	-0.6
2,293,706	+		PP_2019	0.88	-0.8
2,293,706	+		PP_2019	0.88	-2.3
2,293,706	+		PP_2019	0.88	+0.9
2,293,707	-		PP_2019	0.88	-0.9
2,293,707	-		PP_2019	0.88	-0.1
2,293,707	-		PP_2019	0.88	+0.9
2,293,707	-		PP_2019	0.88	-0.6
2,293,707	-		PP_2019	0.88	+0.3
2,293,708	+				+1.2
2,293,712	+		PP_2019	0.88	+1.4
2,293,712	+		PP_2019	0.88	+0.2
2,293,712	+		PP_2019	0.88	+0.5
2,293,712	+		PP_2019	0.88	-0.2
2,293,713	-		PP_2019	0.88	-0.3
2,293,713	-		PP_2019	0.88	+0.3
2,293,713	-		PP_2019	0.88	+0.5
2,293,763	+		PP_2019	0.90	+0.2
2,293,763	+		PP_2019	0.90	+0.6
2,293,767	-				-0.5
2,293,830	+				-0.3
2,293,830	+				-0.4
2,293,830	+				-0.3
2,293,830	+				+0.3
2,293,831	-				+0.7
2,293,831	-				+2.5
2,293,831	-				-1.1
2,294,036	+				-1.7
2,294,038	+				+0.0
2,294,039	-				-0.9
2,294,069	-		PP_2020	0.18	-1.4
2,294,069	-		PP_2020	0.18	-0.3
2,294,069	-		PP_2020	0.18	-0.5
2,294,069	-		PP_2020	0.18	-0.6
2,294,069	-		PP_2020	0.18	-0.2
2,294,104	+		PP_2020	0.28	-1.1
2,294,105	-		PP_2020	0.28	-0.5
2,294,290	+		PP_2020	0.80	-0.5
2,294,290	+		PP_2020	0.80	-1.2
2,294,291	-		PP_2020	0.81	-2.4
2,294,291	-		PP_2020	0.81	-0.2
2,294,291	-		PP_2020	0.81	+0.5
2,294,291	-		PP_2020	0.81	-0.7
2,294,291	-		PP_2020	0.81	-0.6
2,294,291	-		PP_2020	0.81	-1.1
2,294,412	+				-1.8
2,294,413	-				+0.5
2,294,435	+				+0.6
2,294,435	+				+0.1
2,294,439	+		PP_2021	0.10	-0.2
2,294,439	+		PP_2021	0.10	+0.8
2,294,439	+		PP_2021	0.10	+0.6
2,294,439	+		PP_2021	0.10	+0.2
2,294,439	+		PP_2021	0.10	-0.1
2,294,439	+		PP_2021	0.10	+2.5
2,294,439	+		PP_2021	0.10	-0.2
2,294,440	-		PP_2021	0.10	+0.0
2,294,440	-		PP_2021	0.10	-0.2
2,294,440	-		PP_2021	0.10	-2.2
2,294,440	-		PP_2021	0.10	-0.3
2,294,440	-		PP_2021	0.10	-0.3
2,294,685	+		PP_2021	0.32	+0.5
2,294,685	+		PP_2021	0.32	-0.9
2,294,704	-		PP_2021	0.34	-0.1
2,294,704	-		PP_2021	0.34	-0.8
2,294,796	-		PP_2021	0.42	+1.5
2,294,877	+		PP_2021	0.49	-1.5
2,294,878	-		PP_2021	0.49	+0.6
2,294,913	+		PP_2021	0.52	+0.5
2,294,913	+		PP_2021	0.52	+0.2
2,294,914	-		PP_2021	0.52	+0.2
2,294,914	-		PP_2021	0.52	-0.3
2,295,034	-		PP_2021	0.63	+0.7
2,295,186	+		PP_2021	0.76	+0.1
2,295,186	+		PP_2021	0.76	-0.8
2,295,186	+		PP_2021	0.76	-0.5
2,295,187	-		PP_2021	0.76	+1.6