Strain Fitness in Pseudomonas putida KT2440 around PP_1999

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,266,232	+				+0.9
2,266,232	+				-2.0
2,266,232	+				+1.2
2,266,233	-				-0.7
2,266,303	+		PP_1998	0.11	-0.5
2,266,435	+		PP_1998	0.31	-0.1
2,266,436	-		PP_1998	0.31	-0.3
2,266,495	+		PP_1998	0.40	+2.5
2,266,495	+		PP_1998	0.40	-0.9
2,266,717	+		PP_1998	0.75	-0.7
2,266,727	+		PP_1998	0.76	+0.3
2,266,727	+		PP_1998	0.76	-1.1
2,266,727	+		PP_1998	0.76	+0.6
2,266,728	-		PP_1998	0.76	-0.4
2,266,728	-		PP_1998	0.76	+1.5
2,266,728	-				+1.9
2,266,736	+		PP_1998	0.78	+0.7
2,266,736	+		PP_1998	0.78	-0.2
2,266,736	+		PP_1998	0.78	+0.2
2,266,736	+		PP_1998	0.78	+1.0
2,266,737	-		PP_1998	0.78	-0.1
2,266,737	-		PP_1998	0.78	+0.3
2,266,737	-		PP_1998	0.78	-0.6
2,266,738	+		PP_1998	0.78	+0.2
2,266,769	+		PP_1998	0.83	+0.5
2,266,770	-		PP_1998	0.83	-0.1
2,266,931	+				-0.5
2,267,084	+				-0.3
2,267,084	+				-1.2
2,267,232	+		PP_1999	0.28	-0.8
2,267,244	+		PP_1999	0.30	-0.8
2,267,244	+		PP_1999	0.30	-0.5
2,267,941	-		PP_2000	0.18	+0.7
2,268,364	-		PP_2000	0.46	+1.5