Strain Fitness in Pseudomonas putida KT2440 around PP_1995

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,262,250	+				+0.9
2,262,251	-				+0.1
2,262,252	+				-1.2
2,262,252	+				-1.6
2,262,253	-				+2.5
2,262,269	+				+0.6
2,262,365	-				-0.5
2,262,674	-		PP_1994	0.45	+0.5
2,262,682	+		PP_1994	0.46	-0.3
2,262,682	+		PP_1994	0.46	+2.5
2,262,683	-		PP_1994	0.46	+2.5
2,262,683	-				+2.5
2,262,851	-				+0.9
2,262,851	-		PP_1994	0.66	+0.9
2,262,851	-		PP_1994	0.66	+1.5
2,262,851	-		PP_1994	0.66	+3.1
2,262,865	-		PP_1994	0.67	+2.5
2,262,931	-		PP_1994	0.75	+1.5
2,263,052	-				+2.5
2,263,099	+				+2.5
2,263,166	+				-1.4
2,263,166	+				+0.4
2,263,240	+				-3.6
2,263,304	+		PP_1995	0.15	-3.9
2,263,305	-		PP_1995	0.16	+0.9
2,263,452	+		PP_1995	0.39	-3.1
2,263,493	+		PP_1995	0.46	-3.5
2,263,563	-		PP_1995	0.57	-0.5
2,263,595	+		PP_1995	0.62	-2.9
2,263,595	+		PP_1995	0.62	-2.3
2,263,595	+		PP_1995	0.62	-3.3
2,263,595	+		PP_1995	0.62	-3.8
2,263,595	+		PP_1995	0.62	-2.3
2,263,596	-		PP_1995	0.62	-0.8
2,263,596	-		PP_1995	0.62	-1.5
2,263,596	-		PP_1995	0.62	-0.7
2,263,596	-		PP_1995	0.62	-1.3
2,263,596	-		PP_1995	0.62	+0.5
2,263,596	-		PP_1995	0.62	+0.2
2,263,596	-		PP_1995	0.62	-0.3
2,263,596	-		PP_1995	0.62	+2.5
2,263,596	-		PP_1995	0.62	+1.5
2,263,596	-		PP_1995	0.62	-0.1
2,263,596	-		PP_1995	0.62	-1.0
2,263,599	+		PP_1995	0.63	-2.6
2,263,599	+		PP_1995	0.63	-2.2
2,263,733	+		PP_1995	0.85	-2.3
2,263,922	+				-1.0
2,263,922	+				+0.0