Strain Fitness in Pseudomonas putida KT2440 around PP_1988

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,252,780	-				-0.1
2,252,784	-				-0.2
2,253,076	+		PP_1987	0.28	-0.0
2,253,385	+		PP_1987	0.68	-0.3
2,253,555	-				-3.2
2,253,562	+				-0.5
2,253,563	-				+0.5
2,253,563	-				+0.2
2,253,563	-				-3.0
2,253,565	+				+0.7
2,253,752	+				-1.1
2,254,102	+		PP_1988	0.39	-2.3
2,254,156	+		PP_1988	0.44	-4.4
2,254,157	-		PP_1988	0.44	+0.2
2,254,157	-		PP_1988	0.44	-2.1
2,254,157	-		PP_1988	0.44	-2.8
2,254,172	-		PP_1988	0.45	-3.2
2,254,345	+		PP_1988	0.61	-2.3
2,254,345	+		PP_1988	0.61	-1.5
2,254,345	+		PP_1988	0.61	-2.2
2,254,345	+		PP_1988	0.61	-3.7
2,254,346	-		PP_1988	0.61	-3.0
2,254,346	-		PP_1988	0.61	+0.2
2,254,346	-		PP_1988	0.61	-3.3
2,254,346	-		PP_1988	0.61	-2.9
2,254,510	+		PP_1988	0.76	-3.1
2,254,511	-		PP_1988	0.77	-2.1
2,254,511	-		PP_1988	0.77	+2.5
2,254,511	-		PP_1988	0.77	-0.1
2,254,511	-		PP_1988	0.77	-2.2
2,254,511	-		PP_1988	0.77	-2.3
2,254,603	+		PP_1988	0.85	-3.0
2,254,717	-				-0.7
2,254,762	+				+0.4
2,254,762	+				-1.0
2,254,809	+				+0.0
2,254,809	+				+0.2
2,254,820	+				+1.5