Strain Fitness in Pseudomonas putida KT2440 around PP_1864

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
2,084,187	+				+0.8
2,084,187	+				-0.7
2,084,188	-				-0.3
2,084,203	+				+0.2
2,084,203	+				+0.3
2,084,204	-				-0.1
2,084,204	-				+0.2
2,084,204	-				-0.8
2,084,204	-				-2.2
2,084,345	+		PP_1863	0.26	+0.8
2,084,346	-		PP_1863	0.26	-0.3
2,084,346	-		PP_1863	0.26	-1.7
2,084,346	-		PP_1863	0.26	+0.8
2,084,346	-		PP_1863	0.26	+0.6
2,084,352	+		PP_1863	0.27	-0.3
2,084,352	+		PP_1863	0.27	+0.4
2,084,353	-		PP_1863	0.27	-1.1
2,084,612	+		PP_1863	0.57	-0.3
2,084,756	+		PP_1863	0.74	+1.6
2,084,757	-		PP_1863	0.74	-0.8
2,084,768	-		PP_1863	0.75	+1.0
2,084,768	-		PP_1863	0.75	-0.4
2,084,768	-		PP_1863	0.75	+0.7
2,084,815	+		PP_1863	0.81	-0.7
2,084,815	+		PP_1863	0.81	+0.2
2,084,815	+		PP_1863	0.81	+0.4
2,084,816	-		PP_1863	0.81	-0.4
2,084,816	-		PP_1863	0.81	-0.1
2,084,904	+				-0.5
2,084,966	+				+0.3
2,085,121	+				-0.8
2,085,121	+				+0.7
2,085,121	+				-0.7
2,085,121	+				+0.5
2,085,169	+		PP_1864	0.20	-1.0
2,085,169	+		PP_1864	0.20	-0.5
2,085,169	+		PP_1864	0.20	-1.6
2,085,629	+				-0.0
2,085,656	+				+0.1
2,085,727	+				+0.6
2,085,728	-				-1.2
2,085,728	-				-0.6
2,085,728	-				-0.3
2,085,772	-				+0.2