Strain Fitness in Pseudomonas putida KT2440 around PP_1779

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,986,607	+				-0.5
1,986,608	-				-2.5
1,986,608	-				+0.1
1,986,608	-				+0.9
1,986,742	-				+0.9
1,986,766	+				-1.1
1,986,769	-				+0.5
1,986,791	-				-0.4
1,986,860	-		PP_1778	0.14	-2.3
1,986,887	+		PP_1778	0.18	-0.7
1,986,887	+				+2.5
1,986,887	+		PP_1778	0.18	+2.5
1,986,887	+		PP_1778	0.18	+2.5
1,986,887	+		PP_1778	0.18	-1.2
1,986,888	-		PP_1778	0.18	-0.1
1,986,888	-		PP_1778	0.18	+1.5
1,986,909	+		PP_1778	0.21	-0.9
1,986,910	-		PP_1778	0.21	-2.9
1,986,940	+		PP_1778	0.24	-1.6
1,986,940	+		PP_1778	0.24	+0.5
1,986,944	+		PP_1778	0.25	-1.1
1,986,950	+		PP_1778	0.26	+0.9
1,986,960	-		PP_1778	0.27	+2.5
1,987,046	-		PP_1778	0.38	+0.5
1,987,180	-		PP_1778	0.55	-0.5
1,987,236	+		PP_1778	0.62	-0.4
1,987,237	-		PP_1778	0.62	+0.9
1,987,281	+		PP_1778	0.67	+2.5
1,987,282	-		PP_1778	0.67	-2.4
1,987,413	+		PP_1778	0.84	+0.9
1,987,414	-		PP_1778	0.84	-0.5
1,987,533	+				-0.5
1,987,533	+				-1.0
1,987,534	-				-0.9
1,987,534	-				-0.5
1,987,534	-				-1.1
1,987,534	-				-1.2
1,987,534	-				-2.5
1,987,534	-				+1.5
1,987,534	-				-0.5
1,987,536	+				+1.5
1,987,536	+				-0.7
1,987,536	+				-0.5
1,987,536	+				-1.5
1,987,536	+				-1.4
1,987,537	-				-3.5
1,987,889	+		PP_1779	0.29	-0.1
1,987,890	-		PP_1779	0.29	-2.2
1,987,965	+		PP_1779	0.35	-0.4
1,987,966	-		PP_1779	0.35	-3.0
1,987,966	-		PP_1779	0.35	-0.7
1,987,970	-		PP_1779	0.36	-3.2
1,987,970	-		PP_1779	0.36	+0.1
1,988,068	+		PP_1779	0.44	-1.2
1,988,069	-		PP_1779	0.44	+1.5
1,988,069	-		PP_1779	0.44	-0.1
1,988,133	-		PP_1779	0.49	-0.9
1,988,236	+		PP_1779	0.57	+0.1
1,988,237	-		PP_1779	0.57	-2.2
1,988,239	+		PP_1779	0.58	+0.9
1,988,337	+		PP_1779	0.66	-0.5
1,988,357	-		PP_1779	0.67	+0.5
1,988,424	-		PP_1779	0.73	+0.9
1,988,454	-		PP_1779	0.75	-1.7
1,988,454	-		PP_1779	0.75	-1.0
1,988,461	+		PP_1779	0.76	+2.5
1,988,499	+		PP_1779	0.79	-1.5
1,988,650	+				-1.6
1,988,651	-				+0.5
1,988,651	-				-1.1
1,988,651	-				-0.4
1,988,651	-				-0.9
1,988,651	-				-2.2
1,988,691	+				-1.1
1,988,691	+				-0.9
1,988,692	-				-2.9
1,988,766	+				-1.3
1,988,767	-				-1.1
1,988,787	+				+1.0
1,988,829	+				-1.0
1,988,829	+				+0.8
1,988,829	+				+0.2
1,988,829	+				+0.5
1,988,829	+				-1.9
1,988,829	+				-1.4
1,988,829	+				+2.5
1,988,830	-				-1.1
1,988,830	-				-2.9
1,988,830	-				-1.0
1,988,830	-				-0.0
1,988,830	-				-1.1
1,988,830	-				-0.4
1,988,830	-				+1.9
1,988,830	-				+1.5
1,988,831	+				+1.1
1,988,831	+				-0.9
1,988,831	+				-0.4
1,988,832	-				-1.2
1,988,832	-				-0.7
1,988,832	-				-0.8
1,988,832	-				-0.8
1,988,918	-				-1.0
1,989,029	+				-1.0
1,989,029	+				-0.7
1,989,029	+				-0.5
1,989,030	-				-2.8
1,989,128	+				-0.1
1,989,128	+				-0.9
1,989,133	+				+0.9
1,989,134	-				-0.8
1,989,134	-				-2.8
1,989,157	+				+3.5
1,989,233	+		PP_1780	0.10	-2.5
1,989,247	+		PP_1780	0.10	-3.4
1,989,247	+		PP_1780	0.10	-2.1
1,989,247	+		PP_1780	0.10	-1.1
1,989,247	+		PP_1780	0.10	-1.1
1,989,248	-		PP_1780	0.10	+0.9
1,989,248	-		PP_1780	0.10	+0.1
1,989,268	-		PP_1780	0.11	+0.2
1,989,268	-		PP_1780	0.11	-1.1
1,989,365	-		PP_1780	0.13	-0.2
1,989,382	+		PP_1780	0.13	-1.9
1,989,497	+		PP_1780	0.16	-1.0
1,989,557	+		PP_1780	0.17	-0.4
1,989,558	-		PP_1780	0.17	-1.0