Strain Fitness in Pseudomonas putida KT2440 around PP_1716

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,915,429	+		PP_1714	0.81	-0.6
1,915,570	+				-0.0
1,915,570	+				-0.8
1,915,570	+				-0.7
1,915,571	-				+0.5
1,915,571	-				-0.2
1,915,682	+				+0.1
1,915,682	+				+0.8
1,915,683	-				+0.1
1,915,719	-				-0.2
1,915,719	-				-1.2
1,915,827	+				-3.8
1,915,828	-				+0.1
1,915,828	-				+0.0
1,916,168	+		PP_1715	0.70	-1.4
1,916,346	+				-0.2
1,916,347	-				-0.4
1,916,352	-				+2.5
1,916,411	+				+0.5
1,916,418	+				-0.4
1,916,418	+				-0.2
1,916,476	+		PP_1716	0.19	-0.3
1,916,476	+		PP_1716	0.19	-0.9
1,916,477	-		PP_1716	0.19	-2.4
1,916,650	+				+0.1
1,916,836	-		PP_1717	0.24	+2.5
1,917,093	+				+0.3
1,917,094	-				-0.4
1,917,094	-				+0.8
1,917,100	-				+0.6
1,917,136	+				+0.4
1,917,136	+				+1.9
1,917,145	+				+0.1
1,917,145	+				+0.2
1,917,145	+				-0.6
1,917,232	+				+0.0
1,917,244	+				-0.6
1,917,524	+		PP_1718	0.35	-1.1
1,917,525	-		PP_1718	0.35	+1.9
1,917,525	-		PP_1718	0.35	+0.2
1,917,633	+		PP_1718	0.45	-0.6
1,917,633	+		PP_1718	0.45	-0.2
1,917,634	-		PP_1718	0.45	-0.6
1,917,634	-		PP_1718	0.45	-0.5