Strain Fitness in Pseudomonas putida KT2440 around PP_1553

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,751,486	-				-0.3
1,751,506	+				+0.7
1,751,506	+				-0.4
1,751,506	+				+0.6
1,751,506	+				-1.1
1,751,507	-				-0.4
1,751,507	-				-1.1
1,751,507	-				-0.1
1,751,509	+				-1.9
1,751,509	+				-1.6
1,751,509	+				-0.4
1,751,510	-				-0.8
1,751,510	-				+1.2
1,751,733	+		PP_1552	0.20	-0.9
1,751,768	+		PP_1552	0.25	-2.9
1,751,768	+		PP_1552	0.25	-0.8
1,751,769	-		PP_1552	0.25	+0.0
1,751,820	-		PP_1552	0.32	-0.2
1,751,997	-		PP_1552	0.57	+0.7
1,752,222	-		PP_1552	0.89	-0.2
1,752,222	-		PP_1552	0.89	-0.4
1,752,429	-		PP_1553	0.43	-0.6
1,752,432	+		PP_1553	0.44	-1.1
1,752,432	+		PP_1553	0.44	+0.5
1,752,433	-		PP_1553	0.44	-0.5
1,752,709	-		PP_1554	0.27	+0.3
1,752,709	-		PP_1554	0.27	-0.2
1,752,709	-		PP_1554	0.27	+0.6
1,752,848	-		PP_1554	0.60	-0.7
1,753,112	+				+2.5
1,753,190	+		PP_1555	0.12	-0.3
1,753,190	+		PP_1555	0.12	+0.1
1,753,190	+		PP_1555	0.12	-0.3
1,753,191	-		PP_1555	0.13	+1.5
1,753,487	+		PP_1555	0.36	-0.7
1,753,487	+		PP_1555	0.36	-0.0
1,753,565	-		PP_1555	0.42	-0.4