Strain Fitness in Pseudomonas putida KT2440 around PP_1460

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,664,889	+		PP_1459	0.19	+0.1
1,664,993	-				+2.4
1,665,090	-		PP_1459	0.35	-0.7
1,665,090	-		PP_1459	0.35	+0.9
1,665,098	+		PP_1459	0.36	+0.1
1,665,098	+		PP_1459	0.36	+0.1
1,665,099	-		PP_1459	0.36	+0.5
1,665,204	-		PP_1459	0.45	+0.5
1,665,204	-		PP_1459	0.45	-0.1
1,665,578	+		PP_1459	0.76	-1.8
1,665,578	+		PP_1459	0.76	-0.3
1,665,851	+				-1.4
1,665,851	+				+0.2
1,665,883	-				-0.4
1,665,892	+				-1.4
1,666,333	+		PP_1460	0.54	+1.3
1,666,418	-		PP_1460	0.63	-1.4
1,666,418	-		PP_1460	0.63	-0.6
1,666,607	+		PP_1460	0.86	+3.8
1,666,607	+		PP_1460	0.86	-1.1
1,666,619	+		PP_1460	0.87	-1.6
1,666,619	+		PP_1460	0.87	-1.6
1,666,633	+		PP_1460	0.89	+0.2
1,666,633	+		PP_1460	0.89	+0.4
1,666,633	+		PP_1460	0.89	+1.3
1,666,633	+		PP_1460	0.89	-1.9
1,666,633	+		PP_1460	0.89	-1.6
1,666,634	-		PP_1460	0.89	-0.7
1,666,634	-		PP_1460	0.89	-0.8
1,666,634	-		PP_1460	0.89	-1.1
1,666,634	-		PP_1460	0.89	-0.7
1,666,635	+		PP_1460	0.89	-1.7
1,666,635	+		PP_1460	0.89	-0.8
1,666,635	+		PP_1460	0.89	-2.0
1,666,635	+		PP_1460	0.89	-2.4
1,666,635	+		PP_1460	0.89	+0.1
1,666,635	+		PP_1460	0.89	-0.5
1,666,636	-		PP_1460	0.89	-2.6
1,666,636	-		PP_1460	0.89	-1.3
1,666,636	-		PP_1460	0.89	-1.1
1,666,636	-		PP_1460	0.89	-0.2
1,666,636	-		PP_1460	0.89	-2.4
1,666,636	-		PP_1460	0.89	-1.2
1,666,636	-		PP_1460	0.89	-0.1
1,666,636	-		PP_1460	0.89	+1.4
1,666,636	-		PP_1460	0.89	-0.9
1,666,636	-		PP_1460	0.89	-1.2
1,666,637	+		PP_1460	0.89	-0.1
1,666,637	+		PP_1460	0.89	-0.3
1,666,637	+		PP_1460	0.89	-0.2
1,666,637	+		PP_1460	0.89	+0.6
1,666,637	+		PP_1460	0.89	-1.1
1,666,637	+		PP_1460	0.89	-0.8
1,666,637	+		PP_1460	0.89	-0.9
1,666,637	+		PP_1460	0.89	-1.8
1,666,637	+		PP_1460	0.89	+0.1
1,666,637	+		PP_1460	0.89	-0.3
1,666,638	-		PP_1460	0.89	-1.3
1,666,638	-		PP_1460	0.89	+1.4
1,666,638	-		PP_1460	0.89	-2.2
1,666,638	-		PP_1460	0.89	+0.1
1,666,638	-		PP_1460	0.89	-2.8
1,666,638	-		PP_1460	0.89	-1.3
1,666,638	-		PP_1460	0.89	-1.0
1,666,638	-				+0.9
1,666,640	-		PP_1460	0.90	-1.8
1,666,640	-		PP_1460	0.90	-0.7
1,666,686	+				+0.2
1,666,686	+				-0.4
1,666,686	+				-0.1
1,666,686	+				-1.7
1,666,686	+				-0.6
1,666,686	+				-0.7
1,666,687	-				-1.6
1,666,687	-				-0.7
1,666,687	-				-0.9
1,666,687	-				-1.4
1,666,687	-				-0.6
1,666,687	-				+0.1
1,666,687	-				+0.3
1,666,687	-				-0.9
1,666,709	-				-1.5
1,666,729	-				+0.0
1,666,729	-				-0.4
1,666,798	+				+0.1
1,666,875	-				-0.8
1,666,878	+				+0.2
1,666,878	+				-0.5
1,666,878	+				-0.2
1,666,879	-				+0.4
1,666,879	-				-1.0