Strain Fitness in Pseudomonas putida KT2440 around PP_1435

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,635,640	+				-0.3
1,635,673	+				+0.1
1,635,723	-				-0.8
1,635,823	-				+0.4
1,635,851	+				-0.8
1,635,851	+				-0.2
1,635,851	+				-0.2
1,635,852	-				+0.5
1,635,852	-				+0.0
1,635,852	-				-0.4
1,635,856	-				-0.0
1,635,872	+				-0.5
1,635,873	-				+0.8
1,636,154	+		PP_1435	0.48	-1.4
1,636,154	+		PP_1435	0.48	-0.3
1,636,154	+		PP_1435	0.48	-0.5
1,636,154	+		PP_1435	0.48	-0.2
1,636,154	+		PP_1435	0.48	-0.3
1,636,155	-		PP_1435	0.48	+1.5
1,636,155	-		PP_1435	0.48	-0.3
1,636,155	-				+2.5
1,636,155	-		PP_1435	0.48	+0.0
1,636,155	-		PP_1435	0.48	-0.3
1,636,516	+				+0.2
1,636,516	+				-0.3
1,636,516	+				+0.1
1,636,516	+				+0.6
1,636,517	-				+2.0
1,636,517	-				+2.0
1,636,517	-				-0.2
1,636,517	-				-0.2
1,636,517	-				+1.2
1,636,517	-				-1.1
1,637,389	+				-0.5