Strain Fitness in Pseudomonas putida KT2440 around PP_1255

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,432,137	-		PP_1254	0.20	-0.8
1,432,444	+		PP_1254	0.48	-1.0
1,432,445	-		PP_1254	0.48	-0.5
1,432,467	+		PP_1254	0.50	-0.9
1,432,468	-		PP_1254	0.50	-0.0
1,432,926	+				+1.0
1,432,927	-				+0.5
1,432,927	-				-0.1
1,432,927	-				+0.4
1,432,984	-				+1.0
1,433,084	+				-0.3
1,433,138	-				-0.2
1,433,155	+				-0.9
1,433,691	+		PP_1255	0.44	-0.4
1,433,691	+		PP_1255	0.44	-1.3
1,433,817	+		PP_1255	0.55	-2.4
1,434,214	-		PP_1255	0.86	+0.2
1,434,481	-				-0.2
1,434,481	-				+0.7
1,434,619	+		PP_1256	0.15	+0.1
1,434,620	-		PP_1256	0.15	-1.0
1,435,265	-		PP_1256	0.56	+0.8
1,435,265	-		PP_1256	0.56	+0.5