Strain Fitness in Pseudomonas putida KT2440 around PP_1231

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,408,377	+		PP_1230	0.55	-0.5
1,408,377	+		PP_1230	0.55	+1.8
1,408,378	-		PP_1230	0.55	+0.1
1,408,378	-		PP_1230	0.55	+0.6
1,408,395	-		PP_1230	0.56	-0.7
1,408,431	+		PP_1230	0.59	+0.2
1,408,956	-				-0.7
1,408,979	+				-0.3
1,409,099	+				+1.2
1,409,099	+				-0.5
1,409,100	-				+0.3
1,409,117	+				-1.2
1,409,156	+				-1.0
1,409,170	+				-0.6
1,409,170	+				-0.7
1,409,171	-				-0.1
1,409,171	-				+1.1
1,409,171	-				+0.4
1,409,171	-				-0.5
1,409,171	-				-1.1
1,409,192	-				-0.5
1,409,211	+				-0.5
1,409,212	-				-2.1
1,409,212	-				-1.0
1,409,263	-				-0.9
1,409,263	-				+0.5
1,409,288	-				+1.4
1,409,288	-				-0.4
1,409,476	-		PP_1231	0.14	-0.9
1,409,476	-		PP_1231	0.14	-0.9
1,409,478	+		PP_1231	0.14	-0.9
1,409,478	+		PP_1231	0.14	-3.1
1,409,479	-		PP_1231	0.14	+2.4
1,409,479	-		PP_1231	0.14	-2.5
1,409,647	+		PP_1231	0.30	-0.2
1,409,648	-		PP_1231	0.30	-1.6
1,409,749	-		PP_1231	0.39	-0.6
1,409,883	+		PP_1231	0.52	-2.4
1,409,883	+		PP_1231	0.52	+0.8
1,409,883	+		PP_1231	0.52	-3.5
1,409,884	-		PP_1231	0.52	-4.5
1,410,405	+				-0.5
1,410,406	-				-0.2
1,410,434	+				+0.7
1,410,437	-				-1.5
1,410,437	-				-0.0
1,410,437	-				-0.6
1,410,437	-				+1.3
1,410,437	-				+0.0
1,410,448	+				+0.9
1,410,607	+				-0.5
1,410,607	+				+0.2
1,410,643	-		PP_1232	0.10	-1.3
1,410,656	-		PP_1232	0.11	-0.3
1,410,656	-		PP_1232	0.11	-0.9
1,410,656	-		PP_1232	0.11	-0.6
1,410,687	-		PP_1232	0.13	-2.4
1,410,687	-		PP_1232	0.13	+0.2
1,410,687	-		PP_1232	0.13	+1.7
1,410,932	-		PP_1232	0.30	-1.5
1,411,027	+		PP_1232	0.37	-0.0
1,411,028	-		PP_1232	0.37	+1.4
1,411,105	+		PP_1232	0.42	-0.3
1,411,105	+		PP_1232	0.42	-0.2
1,411,106	-		PP_1232	0.42	-0.8
1,411,106	-		PP_1232	0.42	-1.5
1,411,139	-		PP_1232	0.45	+1.1
1,411,187	+		PP_1232	0.48	-0.0
1,411,202	+		PP_1232	0.49	-0.2
1,411,202	+		PP_1232	0.49	-0.6