Strain Fitness in Pseudomonas putida KT2440 around PP_1056

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,203,671	+				+0.2
1,203,671	+				-0.8
1,203,671	+				+0.4
1,204,050	-		PP_1055	0.61	+2.4
1,204,050	-		PP_1055	0.61	-1.9
1,204,301	-				+0.0
1,204,327	+				-1.3
1,204,327	+				-0.0
1,204,328	-				+0.9
1,204,328	-				-0.1
1,204,328	-				+0.4
1,204,331	+				+0.5
1,204,331	+				+1.4
1,204,332	-				-0.7
1,204,332	-				-1.1
1,204,332	-				+0.1
1,204,332	-				-0.1
1,204,332	-				-0.7
1,204,332	-				+0.7
1,204,386	+				+0.7
1,204,386	+				+0.2
1,204,387	-				-0.5
1,204,398	+				+0.8
1,204,496	+				-0.1
1,204,497	-				-1.3
1,204,510	+				-0.3
1,204,510	+				+0.5
1,204,510	+				-1.0
1,204,592	+		PP_1056	0.17	+0.4
1,204,592	+		PP_1056	0.17	+0.8
1,204,593	-		PP_1056	0.17	+0.4
1,204,621	-		PP_1056	0.21	+0.2
1,204,812	-		PP_1056	0.46	-0.4
1,204,977	-		PP_1056	0.68	-0.6
1,204,977	-		PP_1056	0.68	+0.9
1,205,073	+		PP_1056	0.80	+0.6
1,205,074	-		PP_1056	0.80	+0.8
1,205,074	-		PP_1056	0.80	+0.2
1,205,074	-		PP_1056	0.80	-0.6
1,205,074	-		PP_1056	0.80	+0.3
1,205,097	+		PP_1056	0.83	+0.2
1,205,493	+		PP_1057	0.51	+0.1
1,205,493	+		PP_1057	0.51	+0.0
1,205,494	-		PP_1057	0.51	-0.2
1,205,494	-		PP_1057	0.51	+2.4
1,205,494	-		PP_1057	0.51	-1.6
1,205,494	-		PP_1057	0.51	+2.4
1,205,514	-		PP_1057	0.55	-1.1
1,205,514	-		PP_1057	0.55	-1.0
1,205,562	+		PP_1057	0.63	-1.1
1,205,563	-		PP_1057	0.64	-1.3
1,205,563	-		PP_1057	0.64	-0.1
1,205,563	-		PP_1057	0.64	+0.2
1,205,587	-		PP_1057	0.68	+0.6
1,205,587	-		PP_1057	0.68	-0.2
1,205,587	-		PP_1057	0.68	-1.9
1,205,587	-		PP_1057	0.68	+0.3
1,205,587	-		PP_1057	0.68	-0.3
1,205,762	-				-0.7
1,205,814	-				+0.5
1,205,818	-				+0.0