Strain Fitness in Pseudomonas putida KT2440 around PP_1025

Experiment: 40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	40hr timepoint of 1L M9/1% dextrose CONTINUOUS FED BATCH fermentation in 2L Sartorius Biostat, feed rate 2.15g/hr dextrose, set to 30% DO.
1,168,501	-				-0.6
1,168,547	+				-0.7
1,168,547	+				-0.2
1,168,548	-				+0.3
1,168,581	-				-0.3
1,168,592	+				-0.4
1,168,641	-				-1.3
1,168,643	+				+0.8
1,168,643	+				-0.2
1,168,644	-				+0.2
1,168,644	-				-0.1
1,168,644	-				+0.0
1,168,838	+				-0.5
1,168,839	-				-1.2
1,168,872	+				-0.6
1,168,872	+				-2.0
1,168,872	+				-2.6
1,168,873	-				-0.9
1,168,920	+		PP_1025	0.11	+2.8
1,168,920	+		PP_1025	0.11	-0.4
1,169,082	+		PP_1025	0.21	+0.3
1,169,082	+		PP_1025	0.21	-0.7
1,169,082	+		PP_1025	0.21	-1.0
1,169,082	+		PP_1025	0.21	-0.9
1,169,083	-		PP_1025	0.21	-1.2
1,169,103	+		PP_1025	0.22	+2.1
1,169,103	+		PP_1025	0.22	+1.8
1,169,103	+		PP_1025	0.22	+0.8
1,169,103	+		PP_1025	0.22	-1.3
1,169,103	+		PP_1025	0.22	-1.1
1,169,104	-		PP_1025	0.22	-0.4
1,169,104	-		PP_1025	0.22	-1.0
1,169,104	-		PP_1025	0.22	-1.1
1,169,104	-		PP_1025	0.22	-1.1
1,169,124	+		PP_1025	0.23	-3.1
1,169,124	+		PP_1025	0.23	-1.9
1,169,125	-		PP_1025	0.23	-3.9
1,169,224	-		PP_1025	0.29	-1.9
1,169,224	-		PP_1025	0.29	+2.4
1,169,241	+		PP_1025	0.30	-3.2
1,169,241	+		PP_1025	0.30	-3.8
1,169,242	-		PP_1025	0.30	+0.8
1,169,242	-		PP_1025	0.30	-0.4
1,169,292	+		PP_1025	0.33	-3.1
1,169,292	+		PP_1025	0.33	-0.8
1,169,329	-		PP_1025	0.35	-2.1
1,169,430	+		PP_1025	0.41	-3.1
1,169,430	+		PP_1025	0.41	-3.5
1,169,430	+		PP_1025	0.41	-1.7
1,169,430	+		PP_1025	0.41	-2.6
1,169,430	+		PP_1025	0.41	-2.9
1,169,430	+		PP_1025	0.41	-2.9
1,169,431	-		PP_1025	0.42	-3.1
1,169,431	-		PP_1025	0.42	-2.8
1,169,447	+		PP_1025	0.42	-2.6
1,169,532	+		PP_1025	0.48	-1.1
1,169,532	+		PP_1025	0.48	-2.6
1,169,532	+		PP_1025	0.48	-1.5
1,169,532	+		PP_1025	0.48	-1.2
1,169,740	-		PP_1025	0.60	-2.9
1,169,743	+		PP_1025	0.60	-3.2
1,169,743	+		PP_1025	0.60	-2.7
1,169,744	-		PP_1025	0.60	-2.2
1,169,883	+		PP_1025	0.69	-0.7
1,169,922	+		PP_1025	0.71	-1.3
1,169,923	-		PP_1025	0.71	-1.3
1,169,923	-		PP_1025	0.71	-1.3
1,170,021	+		PP_1025	0.77	-3.0
1,170,021	+		PP_1025	0.77	-3.1
1,170,022	-		PP_1025	0.77	-3.8
1,170,022	-		PP_1025	0.77	-4.0
1,170,022	-		PP_1025	0.77	-2.7
1,170,022	-		PP_1025	0.77	-2.5
1,170,266	+				-2.6
1,170,310	+				-2.1
1,170,310	+				-2.9
1,170,311	-				-2.2
1,170,463	-				+0.4
1,170,463	-				+0.7
1,170,463	-				+0.1
1,170,495	+				-3.4
1,170,771	+				-0.6
1,170,772	-				-0.7
1,170,772	-				-0.5
1,170,772	-				+1.0
1,170,772	-				+2.4
1,170,799	+				-0.8
1,171,025	-		PP_1026	0.22	-1.3
1,171,072	-		PP_1026	0.28	+0.8
1,171,371	+		PP_1026	0.64	+0.1
1,171,385	+		PP_1026	0.66	+0.5